Intravital microscopy of leukocyte-endothelial dynamics using the Heidelberg confocal laser microscope in scleritis and allergic conjunctivitis.
Intravital microscopy of leukocyte-endothelial dynamics using the Heidelberg confocal laser microscope in scleritis and allergic conjunctivitis.
复制标题
使用海德堡共聚焦激光显微镜对巩膜炎和过敏性结膜炎进行白细胞内皮动力学的活体显微镜检查。
作者：
L. Lim;Lani T Hoang;T. Wong;S. Planck;M. Ronick;R. R. Gould;W. Mathers;J. Rosenbaum
目的
使用海德堡共聚焦激光显微镜，与使用白色光的串联扫描显微镜相比，提供了更清晰和分辨率的图像，以检查与正常对照相比，在各种眼部炎症性疾病的炎症部位覆盖的人眼血管中的白细胞-内皮细胞滚动和停滞。
方法
使用带有罗斯托克角膜模块附件的海德堡共聚焦激光显微镜（HRT II）在距结膜上皮表面45-120微米的深度扫描健康对照（n=8）和患有活动性前巩膜炎（n=7）或过敏性眼病（n=4）的患者至少5分钟。
结果
与对照组（6+/-6个细胞/mm 2/min; p=0.0002）或过敏性患者（59+/-44个细胞/mm 2/min; p=0.009）相比，巩膜炎患者（534+/-119个细胞/mm 2/min）的滚动白细胞数量显著增加。与对照组相比，在过敏患者中未观察到统计学显著增加（p=0.059）。与过敏性眼病患者或对照组（均=0个细胞/mm 2; p=0.02）相比，巩膜炎患者（56+/-23个细胞/mm 2）中停滞的白细胞数量存在类似的模式。
结论
与轻度过敏性结膜炎患者和对照组相比，巩膜炎患者的眼表血管中滚动和停滞的白细胞数量显著增加。图像质量与此显微镜是上级的扫描显微镜以前的研究。
PURPOSE
To examine leukocyte-endothelial cell rolling and arrest in human ocular vessels overlying sites of inflammation in various ocular inflammatory diseases in comparison to normal controls using the Heidelberg confocal laser microscope, which provides images with greater clarity and resolution than the tandem scanning microscope that uses white light.
METHODS
Healthy controls (n=8) and patients with active anterior scleritis (n=7) or allergic eye disease (n=4) were scanned using the Heidelberg confocal laser microscope (HRT II) with the Rostock cornea module attachment for a minimum of 5 min at a depth of 45-120 microm from the conjunctival epithelial surface.
RESULTS
There was a marked increase in the number of rolling leukocytes in scleritis patients (534+/-119 cells per mm2/min) versus controls (6+/-6 cells per mm2/min; p=0.0002) or allergic patients (59+/-44 cells per mm2/min; p=0.009). No statistically significant increase was seen in allergic patients compared to controls (p=0.059). A similar pattern was seen in the number of arrested leukocytes in patients with scleritis (56+/-23 cells per mm2) in comparison to either those with allergic eye disease or controls (each=0 cells per mm2; p=0.02).
CONCLUSIONS
Patients with scleritis have a significantly increased number of rolling and arrested leukocytes in superficial ocular vessels in comparison to patients with mild allergic conjunctivitis and controls. The image quality with this microscope is superior to prior studies with a scanning microscope.