Hyper-expression of osteocalcin mRNA in odontoblasts of Hyp mice
Hyper-expression of osteocalcin mRNA in odontoblasts of Hyp mice
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DOI：
10.1177/154405910508400115
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发表时间：
2005-01-01
影响因子：
7.6
通讯作者：
Ooshima, T
中科院分区：
文献类型：
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作者：
Onishi, T;Ogawa, T;Ooshima, T
Hyp小鼠是人类X-连锁低磷酸盐血症的鼠同系物，其在骨和牙本质中显示低矿化。在这项研究中，我们测试了Hyp小鼠的缺陷导致牙本质基质蛋白表达改变的假设，这可能与组织中的低矿化变化有关。定量RT-PCR分析表明，Hyp小鼠牙胚样品中的骨钙素基因的表达显着高于野生型小鼠，而骨连接蛋白，骨桥蛋白，牙本质基质蛋白1，和I型胶原蛋白的基因表达在两种类型的小鼠是相似的。此外，培养的Hyp小鼠牙胚样品显示出比野生型小鼠更高的骨钙素基因表达，这与我们的体内分析结果雅阁。这些结果表明，骨钙素mRNA在Hyp小鼠成牙本质细胞中高表达，并可能与牙本质低矿化有关。
The Hyp mouse is a murine homologue of human X-linked hypophosphatemia that displays hypo-mineralization in bone and dentin. In this study, we tested the hypothesis that the defect in Hyp mice leads to alterations in the expression of dentin matrix proteins that may be associated with the hypo-mineralization changes in the tissues. Quantitative RT-PCR analyses showed that expression of the osteocalcin gene in Hyp mice tooth germ samples was significantly higher than in wild-type mice, whereas the gene expressions of osteonectin, osteopontn, dentin matrix protein 1, and type I collagen in both types of mice were similar. Further, cultured Hyp mice tooth germ samples exhibited a higher expression of the osteocalcin gene than did those from wild-type mice, which was in accord with the results of our in vivo analysis. These findings suggest that osteocalcin mRNA is highly expressed in Hyp mice odontoblasts and may be associated with dentin hypo-mineralization.