Crystal structure of RuvC resolvase in complex with Holliday junction substrate.
Crystal structure of RuvC resolvase in complex with Holliday junction substrate.
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DOI：
10.1093/nar/gkt769
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发表时间：
2013-11
影响因子：
14.9
通讯作者：
Nowotny M
中科院分区：
文献类型：
--
作者：
Górecka KM;Komorowska W;Nowotny M
遗传重组的关键中间体是霍利迪连接（HJ），一种四向DNA结构。在重组结束时，HJ被称为解离酶的特异性核酸酶切割。在革兰氏阴性细菌中，这种切割是由RuvC进行的，RuvC是一种属于逆转录病毒整合酶超家族的二聚核酸内切酶。在这里，我们报告的第一个晶体结构的RuvC在复杂的合成HJ解决在3.75毫米的分辨率。复合物中的连接处呈展开的2重对称构象，其中四个臂指向四面体的顶点。两个易分裂的磷酸位于距离链交换点一个核苷酸处，RuvC从小沟侧接近它们。使用基于巯基的位点特异性交联方法验证了在结构中观察到的关键蛋白质-DNA接触。与已知的噬菌体解离酶核酸内切酶I和核酸内切酶VII的复杂结构相比，RuvC结构在底物结合模式和切割位点的位置方面表现出显着的差异。
The key intermediate in genetic recombination is the Holliday junction (HJ), a four-way DNA structure. At the end of recombination, HJs are cleaved by specific nucleases called resolvases. In Gram-negative bacteria, this cleavage is performed by RuvC, a dimeric endonuclease that belongs to the retroviral integrase superfamily. Here, we report the first crystal structure of RuvC in complex with a synthetic HJ solved at 3.75 Å resolution. The junction in the complex is in an unfolded 2-fold symmetrical conformation, in which the four arms point toward the vertices of a tetrahedron. The two scissile phosphates are located one nucleotide from the strand exchange point, and RuvC approaches them from the minor groove side. The key protein–DNA contacts observed in the structure were verified using a thiol-based site-specific cross-linking approach. Compared with known complex structures of the phage resolvases endonuclease I and endonuclease VII, the RuvC structure exhibits striking differences in the mode of substrate binding and location of the cleavage site.
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影响因子：
11.4
作者：
IWASAKI, H;TAKAHAGI, M;SHINAGAWA, H
通讯作者：
SHINAGAWA, H
DOI：
10.1107/s0907444904019158
发表时间：
2004-12-01
影响因子：
2.2
作者：
Emsley, P;Cowtan, K
通讯作者：
Cowtan, K
影响因子：
14.9
作者：
Chan, SN;Vincent, SD;Lloyd, RG
通讯作者：
Lloyd, RG
DOI：
10.1107/s0907444909052925
发表时间：
2010-02
期刊：
Acta crystallographica. Section D, Biological crystallography
影响因子：
--
作者：
Adams PD;Afonine PV;Bunkóczi G;Chen VB;Davis IW;Echols N;Headd JJ;Hung LW;Kapral GJ;Grosse-Kunstleve RW;McCoy AJ;Moriarty NW;Oeffner R;Read RJ;Richardson DC;Richardson JS;Terwilliger TC;Zwart PH
通讯作者：
Zwart PH
影响因子：
14.9
作者：
Chen L;Shi K;Yin Z;Aihara H
通讯作者：
Aihara H