Identification of cDNA clones for ligninase from Phanerochaete chrysosporium using synthetic oligonucleotide probes.
Identification of cDNA clones for ligninase from Phanerochaete chrysosporium using synthetic oligonucleotide probes.
复制标题
使用合成寡核苷酸探针鉴定来自黄孢原毛平革菌的木质素酶 cDNA 克隆。
DOI：
10.1016/0006-291x(86)91127-7
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发表时间：
1986
影响因子：
3.1
通讯作者：
Reddy,CA
中科院分区：
文献类型：
--
作者：
Zhang,YZ;Zylstra,GJ;Olsen,RH;Reddy,CA
通过使用对应于木质素酶胰蛋白酶肽的部分氨基酸序列的三个合成寡核苷酸探针，从代表黄孢原毛平革菌的 6 天木质素降解培养物的 cDNA 文库（构建到大肠杆菌载体 pUC9 的 PstI 位点中）分离木质素酶的四个 cDNA 克隆。三个探针14.1、14.2和25中的每一个代表32个12或14个碱基长的寡核苷酸的混合物。三个 cDNA 克隆与探针 14.1 杂交，但不与探针 25 或 14.2 杂交，但一个 cDNA 克隆与所有三个探针杂交。差异杂交研究表明，这些克隆是 6 天聚 (A) RNA 所特有的，但不是 2 天聚 (A) RNA 所特有的。
Four cDNA clones for ligninase were isolated from the cDNA library (constructed into the PstI site of E. coli vector pUC9) representing 6 day-old lignin degrading culture of Phanerochaete chrysosporium by the use of three synthetic oligonucleotide probes corresponding to partial amino acid sequences of tryptic peptides of the ligninase. Each of the three probes, 14.1, 14.2 and 25, represents a mixture of 32 12-or 14-base long oligonucleotides. Three cDNA clones hybridized with probe 14.1 but not with probe 25 or 14.2, but one cDNA clone hybridized with all of the three probes. Differential hybridization studies showed that these clones are unique to 6-day poly (A) RNA, but not to 2-day poly (A) RNA.