Dose-controlled exposure of A549 epithelial cells at the air-liquid interface to airborne ultrafine carbonaceous particles
Dose-controlled exposure of A549 epithelial cells at the air-liquid interface to airborne ultrafine carbonaceous particles
复制标题
DOI：
10.1016/j.chemosphere.2006.04.035
复制
发表时间：
2006-12-01
期刊：
影响因子：
8.8
通讯作者：
Hofer, T.
中科院分区：
文献类型：
--
作者：
Bitterle, E.;Karg, E.;Hofer, T.
修改了设计用于容纳三种基于膜的细胞培养物的市售灌注室的几何形状，以进行可靠和剂量控制的气液界面（ALI）暴露。将在膜上生长的汇合的A549上皮细胞整合到腔室系统中，并从腔室底部供应培养基。细胞活力不受无颗粒ALI暴露条件的损害。在ALI暴露于过滤空气6小时和随后用肿瘤坏死因子刺激期间，A549细胞的炎性细胞因子白细胞介素6和白细胞介素8的表达与浸没对照相比没有改变，表明细胞保持其功能完整性。通过火花放电以约2 x 106 cm（-3）的稳定浓度产生具有约95 +/- 5 nm的计数中值迁移率直径的超细碳质模型颗粒，并连续监测以准确测定暴露剂量。递送到ALI暴露系统产生了均匀的颗粒沉积在膜上，沉积效率为2%。A549细胞在中等剂量下暴露于该气溶胶6小时，产生的总颗粒沉积为（2.6 +/- 0.4）x 10（8）cm（-2），相当于（87 +/- 23）ng cm（-2）。2.7倍（p
The geometry of commercially available perfusion chambers designed for harbouring three membrane-based cell cultures was modified for reliable and dose-controlled air-liquid interface (ALI) exposures. Confluent A549 epithelial cells grown on membranes were integrated in the chamber system and supplied with medium from the chamber bottom. Cell viability was not impaired by the conditions of ALI exposure without particles. Expression of the inflammatory cytokines interleukin 6 and interleukin 8 by A549 cells during ALI exposure to filtered air for 6 h and subsequent stimulation with tumor necrosis factor was not altered compared to submersed controls, indicating that the cells maintained their functional integrity. Ultrafine carbonaceous model particles with a count median mobility diameter of about 95 +/- 5 nm were produced by spark discharge at a stable concentration of about 2 x 106 cm(-3) and continuously monitored for accurate determination of the exposure dose. Delivery to the ALI exposure system yielded a homogeneous particle deposition over the membranes with a deposition efficiency of 2%. Mid dose exposure of A549 cells to this aerosol for 6 h yielded a total particle deposition of (2.6 +/- 0.4) x 10(8) cm(-2) corresponding to (87 +/- 23) ng cm(-2). The 2.7-fold (p