Neutralizing and infection-enhancing antibody responses to human immunodeficiency virus type 1 in long-term nonprogressors
Neutralizing and infection-enhancing antibody responses to human immunodeficiency virus type 1 in long-term nonprogressors
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DOI：
10.1093/infdis/173.1.60
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发表时间：
1996-01-01
影响因子：
6.4
通讯作者：
Fauci, AS
中科院分区：
文献类型：
--
作者：
Montefiori, DC;Pantaleo, G;Fauci, AS
从人类免疫缺陷病毒1型（HIV-1）感染的长期无进展者（LTNP）和非LTNP的血清抗体进行了评价，在体外病毒中和和感染增强。来自LTNP的血清中HIV-1毒株IIIB和MN的中和抗体平均滴度更高，并且更频繁地中和来自进展者的原代分离株（14.9% vs 1.3%，P = 0.002）。从3例LTNP的外周血单个核细胞和淋巴结中分离出具有复制能力的HIV-1。来自LTNP的所有病毒均具有非合胞体诱导表型，对病毒分离时获得的自体血清的中和具有抗性，并且几乎没有证据表明对异源血清的中和敏感性提高。HIV-1（IIIB）和原代分离株的补体介导的抗体依赖性增强（C '-ADE）在LTNP和非LTNP血清中同样普遍。结果表明，LTNP产生强烈的血清抗体应答，长期非进展与同源中和或C '-ADE的缺乏无关。
Serum antibodies from human immunodeficiency virus type 1 (HIV-1)-infected long-term nonprogressors (LTNPs) and non-LTNPs were evaluated for virus neutralization and infection enhancement in vitro. Sera from LTNPs had higher average titers of neutralizing antibodies to HIV-1 strains IIIB and MN and more frequently neutralized primary isolates from progressors (14.9% vs, 1.3%, P = .002). Replication-competent HIV-1 was isolated from peripheral blood mononuclear cells and lymph nodes of 3 LTNPs. All viruses from LTNPs had a non-syncytium-inducing phenotype, were resistant to neutralization by autologous serum obtained at the time of virus isolation, and showed little evidence of a heightened sensitivity to neutralization by heterologous sera. Complement-mediated, antibody-dependent enhancement (C'-ADE) of HIV-1(IIIB) and primary isolates was equally prevalent for sera from LTNPs and non-LTNPs. Results indicate that LTNPs produce vigorous serum antibody responses and that long-term nonprogression is not associated with homologous neutralization or the absence of C'-ADE.