New microtome and sectioning method for electron microscopy
New microtome and sectioning method for electron microscopy
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DOI：
10.1126/science.98.2551.455
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发表时间：
1943-01-01
期刊：
影响因子：
56.9
通讯作者：
McKinley, G. M.
中科院分区：
文献类型：
--
作者：
O'Brien, H. C;McKinley, G. M.
为了通过电子束穿透和分辨结构细节，截面的厚度必须约为0.1u。在这个新的切片机中，样品不断地向以10,000 r.pm旋转的旋转刀移动。或者更快地[长破折号]，尖端以每分钟10万英寸的速度移动。在切割刃以这样的速度移动的情况下，与光学显微镜的技术相比，材料可以在不嵌入的情况下被分割成整体，或者在更软的介质中分割，或者冻结到较小的程度。用高速刀具切割的0.1[亩]节以切线形式飞出，分散在空气中。它们可以收集在火棉胶片或其他胶片上，这些胶片或其他胶片安装在靠近刀的通常电子显微镜屏幕上。|摘要作者：M·A·雷恩斯
For penetration and resolution of structural detail by the electron beam, the thickness of the section must be about 0.1 u. In this new microtome the specimen is constantly moved toward a rotary knife revolving at 10,000 r.p.m. or faster[long dash]the cutting edge traveling about 100,000 inches per minute. Where the cutting edge moves at such a speed, the material may be sectioned in toto without embedding, or sectioned in softer media, or frozen to lesser degree, than in the techniques for the light microscope. The 0.1-[mu] sections cut with the high-speed knife fly out at a tangent and are dispersed in the air. They may be collected on collodion films or other films mounted on the usual electron microscope screens held near the knife. || ABSTRACT AUTHORS: M. A. Raines