Fiber reinforced GelMA hydrogel to induce the regeneration of corneal stroma
Fiber reinforced GelMA hydrogel to induce the regeneration of corneal stroma
复制标题
纤维增强凝胶诱导角膜基质再生
DOI：
10.1038/s41467-020-14887-9
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发表时间：
2020-03-18
影响因子：
16.6
通讯作者：
Mi, Shengli
中科院分区：
文献类型：
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作者：
Kong, Bin;Chen, Yun;Mi, Shengli
由于角膜基质复杂的结构和角质细胞-成纤维细胞的转化，其再生一直是一个挑战。在这项研究中，我们制作了网格聚（epsilon -己内酯）-聚（乙二醇）微纤维支架，并在支架中注入甲基丙烯酸明胶（GelMA）水凝胶，获得了三维纤维水凝胶结构；调整纤维间距以制造模拟基质结构的最佳结构，其特性与天然角膜最相似。通过拓扑结构（3d纤维水凝胶、3d凝胶和2d培养皿）和化学因子（血清、抗坏血酸、胰岛素和β -FGF）对角膜基质干细胞向角化细胞或成纤维细胞分化、表型维持、体外和体内组织再生的影响进行研究。结果表明，纤维水凝胶和无血清培养基协同作用，为维持角膜细胞表型和受损角膜基质的再生提供了最佳环境。由于角膜基质结构复杂且易于转化，其再生一直是一个挑战。在这里，作者使用正交排列纤维增强的水凝胶和无血清培养基来维持体内基质再生的角质细胞表型。
Regeneration of corneal stroma has always been a challenge due to its sophisticated structure and keratocyte-fibroblast transformation. In this study, we fabricate grid poly (epsilon -caprolactone)-poly (ethylene glycol) microfibrous scaffold and infuse the scaffold with gelatin methacrylate (GelMA) hydrogel to obtain a 3 D fiber hydrogel construct; the fiber spacing is adjusted to fabricate optimal construct that simulates the stromal structure with properties most similar to the native cornea. The topological structure (3 D fiber hydrogel, 3 D GelMA hydrogel, and 2 D culture dish) and chemical factors (serum, ascorbic acid, insulin, and beta -FGF) are examined to study their effects on the differentiation of limbal stromal stem cells to keratocytes or fibroblasts and the phenotype maintenance, in vitro and in vivo tissue regeneration. The results demonstrate that fiber hydrogel and serum-free media synergize to provide an optimal environment for the maintenance of keratocyte phenotype and the regeneration of damaged corneal stroma. Regeneration of corneal stroma has been a challenge due to its sophisticated structure and the easy transformation of the keratocyte. Here, the authors use a hydrogel reinforced with orthogonally aligned fibres and serum free medium to maintain keratocyte phenotype for the in vivo stromal regeneration.