Lens-less holographic microscopy with high resolving power for 4D measurement of microorganism swimming in water
Lens-less holographic microscopy with high resolving power for 4D measurement of microorganism swimming in water
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具有高分辨率的无透镜全息显微镜，用于对水中游动的微生物进行 4D 测量
DOI：
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发表时间：
2012
期刊：
影响因子：
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通讯作者：
Kunihiro Sato
中科院分区：
文献类型：
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作者：
M. Otani;Kunihiro Sato
提出了一种无透镜全息显微术，用于观察高分辨率的4-D（时空）图像。利用单次激发数字全息技术，从大的离轴全息图中提取出大数值孔径的复振幅同轴全息图。提出了一种从大数值孔径同轴全息图中快速数值再现精确三维像的角谱方法。利用全息显微术记录和再现了无畸变、分辨率大于1μm、深度大于1 mm的三维强度和相位像。论证了实现水中游动微生物四维测量的可能性。
A lens-less holographic microscopy is developed for observation of high-resolution 4-D (spatio-temporal) images. A complex-amplitude in-line hologram with a large numerical aperture is extracted from a large off-axis hologram by applying one-shot digital holography. An angular spectrum method is developed for fast numerical reconstruction of precise 3-D image from the in-line hologram with a large numerical aperture. 3-D intensity and phase images with no distortion, with a resolution higher than 1μm, and with a depth larger than 1mm are recorded and reconstructed by the present holographic microscopy. Possibility is demonstrated to realize 4-D measurement of microorganisms swimming in water.