UNIFOLIATA regulates leaf and flower morphogenesis in pea
UNIFOLIATA regulates leaf and flower morphogenesis in pea
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DOI：
10.1016/s0960-9822(06)00257-0
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发表时间：
1997-08-01
期刊：
影响因子：
9.2
通讯作者：
Ellis, N
中科院分区：
文献类型：
--
作者：
Hofer, J;Turner, L;Ellis, N
背景资料：豌豆突变体unifoliata（uni）的营养表型是将野生型复叶简化为单个小叶，突变体uni植物也是自交不育的，并且花类似于已知的花分生组织和器官特性突变体。在金鱼草和拟南芥中，花分生组织身份基因FLORICAULAILEAFY（FLO/LFY）的突变只影响花的发育，而烟草FLO/LFY同源物NFL在营养组织中表达，这表明NFL在花和叶的祖细胞中指定了确定性。结果：在重组-近交作图群体中，FLO/LFY的豌豆同源基因PEAFLO定位在uni位点上，在分离的同胞群体中，PEAFLO与uni共分离。两个自发的uni突变等位基因的特征，一个在PEAFLO编码序列中含有缺失，另一个在PEAFLO编码序列中含有点突变，预测PEAFLO对应于UNI，并且突变的营养表型由缺陷的PEAFLO基因赋予。uni突变体表明在叶和花的形态发生中存在共同的调控过程，并且花分生组织身份基因具有扩展的作用在植物发育过程中，多效调节基因如UNI支持了叶和花起源于一个共同的祖先孢子叶样结构的假说。UNI对叶和花形态发生过程中的不确定性的调节可能反映了该基因在前被子植物时代的原始功能。
Background: The vegetative phenotype of the pea mutant unifoliata (uni) is a simplification of the wild-type compound leaf to a single leaflet, Mutant uni plants are also self-sterile and the flowers resemble known floral meristem and organ identity mutants. In Antirrhinum and Arabidopsis, mutations in the floral meristem identity gene FLORICAULAILEAFY (FLO/LFY) affect flower development alone, whereas the tobacco FLO/LFY homologue, NFL, is expressed in vegetative tissues, suggesting that NFL specifies determinacy in the progenitor cells for both flowers and leaves. In this paper, we characterised the pea homologue of FLO/LFY, Results: The pea cDNA homologue of FLO/LFY, PEAFLO, mapped to the uni locus in recombinant-inbred mapping populations and markers based on PEAFLO cosegregated with uni in segregating sibling populations. The characterisation of two spontaneous uni mutant alleles, one containing a deletion and the other a point mutation in the PEAFLO coding sequences, predicted that PEAFLO corresponds to UNI and that the mutant vegetative phenotype was conferred by the defective PEAFLO gene,Conclusions: The uni mutant demonstrates that there are shared regulatory processes in the morphogenesis of leaves and flowers and that floral meristem identity genes have an extended role in plant development, Pleiotropic regulatory genes such as UNI support the hypothesis that leaves and flowers derive from a common ancestral sporophyll-like structure, The regulation of indeterminacy during leaf and flower morphogenesis by UNI may reflect a primitive function for the gene in the pre-angiosperm era.