Optimization of simultaneous saccharification and fermentation conditions with amphipathic lignin derivatives for concentrated bioethanol production
Optimization of simultaneous saccharification and fermentation conditions with amphipathic lignin derivatives for concentrated bioethanol production
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DOI：
10.1016/j.biortech.2017.02.018
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发表时间：
2017-05-01
影响因子：
11.4
通讯作者：
Uraki, Yasumitsu
中科院分区：
文献类型：
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作者：
Cheng, Ningning;Koda, Keiichi;Uraki, Yasumitsu
以杉木碱浆黑液为原料制备的两亲性木质素衍生物（a - lds）是间歇加料同步酶糖化发酵（SSF）工艺生产生物乙醇的强力促进剂。为了提高生物乙醇的生产浓度，对反应温度、搅拌程序和a - ld的添加量等条件进行了优化。在38℃条件下，3.0 g/L a - ld存在时，补料SSF的乙醇产量最大，酶回收率高。此外，为了在高底物负荷下实现严格的混合，为1.5 l规模的进料批式SSF设计了配备强力机械搅拌器的罐式发酵罐。最后，在优化条件下，底物负载为30% （w/v）的1.5 L进料批SSF在a - ld存在下可产生87.9 g/L的高乙醇浓度。(C) 2017年作者。Elsevier Ltd.出版。这是一篇基于CC BY-NC-ND许可（http://creativecommons.org/licenses/by-nc-nd/4.0/）的开放获取文章。
Amphipathic lignin derivatives (A-LDs) prepared from the black liquor of soda pulping of Japanese cedar are strong accelerators for bioethanol production under a fed-batch simultaneous enzymatic saccharification and fermentation (SSF) process. To improve the bioethanol production concentration, conditions such as reaction temperature, stirring program, and A-LDs loadings were optimized in both small scale and large scale fed-batch SSF. The fed-batch SSF in the presence of 3.0 g/L A-LDs at 38 degrees C gave the maximum ethanol production and a high enzyme recovery rate. Furthermore, a jar-fermenter equipped with a powerful mechanical stirrer was designed for 1.5 L-scale fed-batch SSF to achieve rigorous mixing during high substrate loading. Finally, the 1.5 L fed-batch SSF with a substrate loading of 30% (w/v) produced a high ethanol concentration of 87.9 g/L in the presence of A-LDs under optimized conditions. (C) 2017 The Authors. Published by Elsevier Ltd. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).