Anisotropic Materials for Skeletal-Muscle-Tissue Engineering.
Anisotropic Materials for Skeletal-Muscle-Tissue Engineering.
复制标题
骨骼肌肉组织工程的各向异性材料。
DOI：
10.1002/adma.201600240
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发表时间：
2016-12
期刊：
影响因子：
--
通讯作者：
Zhang M
中科院分区：
文献类型：
--
作者：
Jana S;Levengood SK;Zhang M
受损骨骼肌组织的修复受到天然组织再生能力的限制。目前的临床方法对于大体积骨骼肌损失的治疗不是最佳的。作为一种替代方法，组织工程是一种很有前途的方法，为受损肌肉组织的功能恢复。典型的组织工程过程涉及支架的设计和制造，该支架紧密模拟天然骨骼肌细胞外基质，允许将细胞组织成生理相关的3D结构。特别地，可以使用各种生物相容性材料来制造模仿天然骨骼肌ECM的形态的各向异性材料，以引导细胞排列、伸长、增殖和分化成肌管。在这篇文章中，我们首先提供了一个与肌肉组织工程的基本概念和肌肉组织工程方法的现状的概述。然后，我们回顾了最近的进展，各向异性支架与微米或纳米尺度的功能和研究如何支架地形，机械和生化线索与观察到的细胞功能和表型的发展。最后，我们强调了一些最新的发展，在设计和应用的各向异性材料在骨骼肌组织工程沿着与他们的潜在影响，在未来的研究和临床应用。本文综述了肌肉组织工程支架材料的研究进展，包括微图案化基质、定向微孔和定向纤维支架。工程对齐矩阵相关的挑战突出和支架地形，机械和生化线索细胞功能和肌原性表型发展的相关性进行了讨论。
Repair of damaged skeletal muscle tissue is limited by the regenerative capacity of native tissue. Current clinical approaches are not optimal for treatment of large volumetric skeletal muscle loss. As an alternative, tissue engineering represents a promising approach for the functional restoration of damaged muscle tissue. A typical tissue engineering process involves the design and fabrication of a scaffold that closely mimics the native skeletal muscle extracellular matrix allowing for organization of cells into a physiologically relevant, 3D architecture. In particular, anisotropic materials, which mimic the morphology of the native skeletal muscle ECM, can be fabricated using various biocompatible materialsto guide cell alignment, elongation, proliferation and differentiation into myotubes. In this article, we first provide an overview of fundamental concepts associated with muscle tissue engineering and the current status of the muscle tissue engineering approaches. We then review recent advances in development of anisotropic scaffolds with micro- or nano-scale features and examine how scaffold topographical, mechanical, and biochemical cues correlate to observed cellular function and phenotype development. Finally, we highlight some recent developments in both the design and utility of anisotropic materials in skeletal muscle tissue engineering along with their potential impact on future research and clinical application. Muscle tissue engineering approaches are reviewed focusing on anisotropic matrices including micropatternered substrates, aligned microporous and aligned fibrous scaffolds. Challenges associated with engineering aligned matrices are highlighted and correlation of scaffold topographical, mechanical and biochemical cues to cellular function and myogenic phenotype development is discussed.