Effects of bone wax on bacterial clearance.
Effects of bone wax on bacterial clearance.
复制标题
骨蜡对细菌清除的影响。
作者：
P. Johnson;D. Fromm
在松质骨中放置骨蜡的止血效果是以骨蜡在骨质部位持续多年为代价的。因此，骨蜡看起来就像是异物。这项研究考察了骨蜡对松质骨清除细菌能力的影响。用18号针将兔的髂骨穿透至标准深度。随后，将无菌或金黄色葡萄球菌(10(5)个菌落形成单位)接种到针头部位，然后将骨蜡圆柱体或类似大小的不锈钢棒放置在皮下。10天后，将骨穿透部位切除并培养。将细菌和骨蜡一起植入骨骼的动物中，80%的动物培养呈阳性。在骨骼被植入细菌和钢棒的动物中，有40%的动物培养呈阳性。这些组之间的差异(使用骨蜡的细菌或使用钢棒的细菌)在统计学上没有显著差异。然而，这些细菌和异物的组合与单纯细菌组、单纯骨蜡组和单纯钢棒组有显著差异，在这些组中没有观察到阳性培养。结果表明，骨蜡对兔松质骨清除标准接种物金黄色葡萄球菌的能力有显著影响。这种影响似乎与使用不同但大小相同的异物(钢棒)观察到的效果相同。
The hemostatic benefit of bone wax placed in cancellous bone occurs at the expense of the wax persisting at the bony site for years. As such, bone wax appears to act as a foreign body. This study examines the effect of bone wax on the ability of cancellous bone to clear bacteria. The iliac crest of rabbits was penetrated to a standard depth with an 18-gauge needle. Subsequently, nothing or Staphylococcus aureus, 10(5) colony-forming units, was innoculated in the needle site and followed by the subcortical placement of a cylinder of bone wax or similarly sized stainless steel rod. Ten days later, the site of bony penetration was excised and cultured. Eighty percent of animals whose bone was implanted with bacteria and bone wax together had positive cultures. Forty percent of animals whose bone was implanted with bacteria and a steel rod also had positive cultures. The difference between these groups (bacteria with bone wax or with steel rod) was not statistically significant. However, these combinations of bacteria with foreign body were significantly different from the bacteria only or bone wax only or steel rod only groups in which no positive cultures were observed. The data indicate that bone wax significantly impairs the ability of cancellous bone of rabbits to clear a standard inoculum of S. aureus. This effect appears to be the same as that observed with a different but similarly sized foreign body (steel rod).