DNA origami tubes with reconfigurable cross-sections
DNA origami tubes with reconfigurable cross-sections
复制标题
具有可重构横截面的 DNA 折纸管
DOI：
10.1039/d2nr05416g
复制
发表时间：
2023
期刊：
影响因子：
6.7
通讯作者：
Castro, Carlos E.
中科院分区：
文献类型：
--
作者：
Kucinic, Anjelica;Huang, Chao-Min;Wang, Jingyuan;Su, Hai-Jun;Castro, Carlos E.
结构 DNA 纳米技术能够设计和构建具有精确几何形状和可编程动态和机械特性的复杂纳米级结构。最近的努力在驱动 DNA 折纸装置的形状变化以及将 DNA 折纸整合到更大的组件中的能力方面取得了重大进展，这开启了使用 DNA 设计形状变形组件作为微型可重构或传感材料的组件的前景。事实上，一些研究已经构建了具有可重构设备的更高阶组件。然而，这些演示使用了运动相对简单的结构，主要是打开和关闭的铰链。为了提高 DNA 折纸组件的形状改变能力，我们开发了一种多组件 DNA 折纸 6 杆机构，该机构可以重新配置成各种形状，并可以合并到更大的组件中，同时保持各种形状转换的能力。我们演示了将 6 杆机构折叠成四种不同的形状，并演示了这些形状之间的多种过渡。我们还研究了竞争性折叠反应中 6 杆机构的形状偏好，以深入了解形状的相对自由能。此外，我们将 6 杆机构聚合成具有各种横截面的管子，由各个机构的形状定义，并且我们展示了改变管子横截面形状的能力。这种将当前单设备重新配置扩展到更高阶尺度的做法为纳米级到微米级 DNA 纳米技术应用（例如生物传感或具有可调特性的材料）奠定了基础。
Structural DNA nanotechnology has enabled the design and construction of complex nanoscale structures with precise geometry and programmable dynamic and mechanical properties. Recent efforts have led to major advances in the capacity to actuate shape changes of DNA origami devices and incorporate DNA origami into larger assemblies, which open the prospect of using DNA to design shape-morphing assemblies as components of micro-scale reconfigurable or sensing materials. Indeed, a few studies have constructed higher order assemblies with reconfigurable devices; however, these demonstrations have utilized structures with relatively simple motion, primarily hinges that open and close. To advance the shape changing capabilities of DNA origami assemblies, we developed a multi-component DNA origami 6-bar mechanism that can be reconfigured into various shapes and can be incorporated into larger assemblies while maintaining capabilities for a variety of shape transformations. We demonstrate the folding of the 6-bar mechanism into four different shapes and demonstrate multiple transitions between these shapes. We also studied the shape preferences of the 6-bar mechanism in competitive folding reactions to gain insight into the relative free energies of the shapes. Furthermore, we polymerized the 6-bar mechanism into tubes with various cross-sections, defined by the shape of the individual mechanism, and we demonstrate the ability to change the shape of the tube cross-section. This expansion of current single-device reconfiguration to higher order scales provides a foundation for nano to micron scale DNA nanotechnology applications such as biosensing or materials with tunable properties.