Measuring Microenvironment Mechanical Stress of Rat Liver During Diethylnitrosamine Induced Hepatocarcinogenesis by Atomic Force Microscope
Measuring Microenvironment Mechanical Stress of Rat Liver During Diethylnitrosamine Induced Hepatocarcinogenesis by Atomic Force Microscope
复制标题
原子力显微镜测量二乙基亚硝胺诱导肝癌过程中大鼠肝脏微环境机械应力
DOI：
10.1002/jemt.20716
复制
发表时间：
2009-09-01
影响因子：
2.5
通讯作者：
Wang, Chunyou
中科院分区：
文献类型：
--
作者：
Gang, Zhao;Qi, Qin;Wang, Chunyou
我们开发了一种高灵敏度的原子力显微镜(AFM)检测肝组织硬度的方法，并研究了肝癌发生的物理特征。Wistar大鼠每周接受二乙基亚硝胺(DEN)或生理盐水(对照组)的腹膜内注射，然后进行为期两周的洗涤。分别于第10、14、18周采集肝脏标本进行病理检查和应激检测。以前正常的肝组织在注射DEN后出现纤维化和癌变。正常组织(生理盐水0.18+/-0.04 Mpa)、肝纤维化组织(8周DEN；0.25+/-0.06 Mpa)和肝硬变(12周DEN；0.39+/-0.06 Mpa)组织的弹性模量(E)值有显著差异，而肝硬变组织和肝细胞癌组织(16周DEN；0.42+/-0.07 Mpa)的E值无显著差异。因此，在肝癌发生过程中，组织硬度定量增加，AFM可以在显微镜水平上灵敏而准确地检测肝脏硬度。Microsc.技术资源。2009年，72：672-678。(C)2009年Wiley-Liss。Inc.
We developed a highly sensitive method to detect liver tissue stiffness with atomic force microscopy (AFM), and investigated the physical features of hepatocarcinogenesis. Wistar rats received weekly intraperitoneal injections of diethylnitrosamine (DEN) or saline (control) followed by a 2-week wash-out period. Liver samples were harvested at 10, 14, or 18 weeks for pathological examination and stress detection. Previously normal liver tissues developed fibrosis and carcinoma after DEN administration. Although the elastic modulus (E) values of the normal (saline; 0.18 +/- 0.04 MPa), fibrotic (8 weeks DEN; 0.25 +/- 0.06 MPa) and cirrhotic (12 weeks DEN; 0.39 +/- 0.06 MPa) tissues were significantly different, there was no significant difference between the E values of the cirrhotic and the hepatic cell carcinoma (16 weeks DEN; 0.42 +/- 0.07 MPa) tissues. Thus, tissue stiffness quantitatively increases during hepatocarcinogenesis, and AFM can be used to sensitively and precisely detect liver stiffness at the microscopic level. Microsc. Res. Tech. 72:672-678, 2009. (C) 2009 Wiley-Liss. Inc.