Genetic evidence of clonal descent of Escherichia coli O157:H7 associated with hemorrhagic colitis and hemolytic uremic syndrome.
Genetic evidence of clonal descent of Escherichia coli O157:H7 associated with hemorrhagic colitis and hemolytic uremic syndrome.
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大肠杆菌 O157:H7 克隆遗传与出血性结肠炎和溶血性尿毒症综合征相关的遗传证据。
DOI：
10.1093/infdis/157.6.1124
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发表时间：
1988
期刊：
影响因子：
--
通讯作者：
Wilson,RA
中科院分区：
文献类型：
--
作者：
Whittam,TS;Wachsmuth,IK;Wilson,RA
用多位点酶电泳法检测了100株大肠埃希菌的17个酶编码位点的等位基因变异，并根据染色体基因型对这些大肠埃希菌的遗传相关性进行了测定。17个位点中有15个位点表现多态性，平均每个位点有3.5个等位基因。比较菌株间观察到的等位基因组合，发现25个不同的多位点基因型，用于定义自然发生的细胞谱系或克隆。聚类分析的基因型数据显示，血清型O157：H7的菌株落入一个明确定义的克隆基因型，共享等位基因，平均90%的酶位点。O157：H7克隆群与其它血清型的产维罗毒素菌株仅为远亲。杆菌这些结果有力地支持了E. coliO157：H7从地理上分离的出血性结肠炎和溶血性尿毒综合征的暴发和散发病例中获得，属于整个北美发生的致病性克隆。
Genetic relatedness of 100 strains ofEscherichia coli, isolated mostly from patients with hemorrhagic colitis or hemolytic uremic syndrome, was determined for chromosomal genotypes on the basis of allelic variation at 17 enzyme-encoding loci detected by multilocus enzyme electrophoresis. Fifteen of the 17 loci were polymorphic, with an average of 3.5 alleles per locus. Comparison of the observed combinations of alleles among strains revealed 25 distinct multilocus genotypes, which were used to define naturally occurring cell lineages or clones. Cluster analysis of the genotypic data revealed that isolates of serotype O157:H7 fall into a well-defined group of clonal genotypes that share alleles, on average,at 90% of their enzyme loci. The O157:H7 clonal group is only distantly related to other Verotoxin-producing strains belonging to other serotypes ofE. coli. The results strongly support the hypothesis that isolates ofE. coliO157:H7 obtained from geographically separate outbreaks and sporadic cases of hemorrhagic colitis and hemolytic uremic syndrome belong to a pathogenic clone that occurs throughout North America.