DETECTION OF CHLAMYDIA-PNEUMONIAE IN AORTIC LESIONS OF ATHEROSCLEROSIS BY IMMUNOCYTOCHEMICAL STAIN
DETECTION OF CHLAMYDIA-PNEUMONIAE IN AORTIC LESIONS OF ATHEROSCLEROSIS BY IMMUNOCYTOCHEMICAL STAIN
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DOI：
10.1161/01.atv.13.10.1501
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发表时间：
1993-10-01
期刊：
影响因子：
--
通讯作者：
GRAYSTON, JT
中科院分区：
文献类型：
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作者：
KUO, CC;GOWN, AM;GRAYSTON, JT
最近的证据表明，南非尸检患者冠状动脉粥样硬化中存在肺炎衣原体抗原和核酸。在这项研究中，免疫细胞化学技术被用于在华盛顿大学回顾性主动脉粥样硬化研究的尸检患者的主动脉粥样硬化中证明肺炎C抗原。患者年龄34 ~ 58岁。衣原体特异性单克隆抗体免疫过氧化物酶染色显示4个脂肪条纹中的1个和17个纤维斑块中的6个对肺炎C抗原阳性；4个对照主动脉组织呈阴性。两个阳性斑块来自同一个病人。使用衣原体和组织类型特异性单克隆抗体的双标记免疫细胞化学染色表明，在动脉粥样硬化病变的巨噬细胞和平滑肌细胞的细胞质中存在抗原。这项研究表明，肺炎C菌在动脉系统动脉粥样硬化病变中的参与范围比以往文献记载的要广泛。
Recent evidence has shown the presence of Chlamydia pneumoniae antigens and nucleic acid in coronary artery atheromas from autopsy patients in South Africa. In this study, the immunocytochemical technique was used to demonstrate C pneumoniae antigens in atheromas of the aorta in autopsy patients from retrospective aortic atherosclerosis studies at the University of Washington. The patients were 34 to 58 years old. Immunoperoxidase staining using Chlamydia-specific monoclonal antibodies showed one of four fatty streaks and six of 17 fibrous plaques were positive for C pneumoniae antigens; four control aortic tissues were negative. Two of the positive plaques were from the same patient. Double-label immunocytochemical staining using Chlamydia- and tissue type-specific monoclonal antibodies demonstrated the antigens in the cytoplasm of macrophages and smooth muscle cells in the atheromatous lesion. This study suggested a wider involvement of C pneumoniae organisms in atherosclerotic lesions of the arterial system than has previously been documented.