Compilation of DNA strand exchange sites for non-homologous recombination in somatic cells.
Compilation of DNA strand exchange sites for non-homologous recombination in somatic cells.
复制标题
体细胞非同源重组 DNA 链交换位点的编译。
DOI：
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发表时间：
1988
影响因子：
14.9
通讯作者：
Andrzej K. Konopka
中科院分区：
文献类型：
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作者：
Andrzej K. Konopka
对496个体细胞非正常杂交区的DNA序列进行了整理和分析。将转染到哺乳动物细胞中的线性DNA上的非同源重组位点（转染的线性DNA; IRR）与剩余的非法重组区（IRR）分开分析。在92%的IRR和93%的TLD中，在体外优先被大鼠肝脏拓扑异构酶I切割的三核苷酸（CAT、CTY、GTY、RAT，其中R =嘌呤，Y =嘧啶）存在于交换位点的10个碱基对（bp）附近。在39%的IRR和38%的IRR中观察到这些三核苷酸的多个重复。在26%的IRR和14%的IRR中发现了5个或更多连续的嘌呤（或互补链上的嘧啶）。14%的IRR和21%的IRR中存在5个或5个以上碱基的腺嘌呤-胸腺嘧啶富集区。11%的IRR中发现了长度超过4个核苷酸的交替嘌呤-嘧啶轨道，但只有4%的IRR中发现了。我讨论了这些研究结果可能的生物学意义，并提出了一个附录，包含在10 bp附近的非同源重组位点分析的序列。
DNA sequences of 496 somatic cell illegitimate crossing over regions were compiled and analyzed. Sites for non-homologous recombination on linear DNAs transfected into mammalian cells (Transfected Linear DNAs; TLD) were analyzed separately from the remaining illegitimate recombination regions (IRR). Trinucleotides that are preferentially cleaved by rat liver topoisomerase I in vitro (CAT, CTY, GTY, RAT where R = purine, Y = pyrimidine) were present in the 10 base pair (bp) vicinity of the cross-over sites in 92% of IRR and 93% of TLD. Multiple repeats of these trinucleotides have been observed in 39% of IRR and 38% of TLD. Runs of five or more contiguous purines (or pyrimidines on the complementary strand) were found in 26% of IRR and 14% of TLD. Adenine-Thymine rich regions of five or more bases were found in 14% of IRR and 21% of TLD. Alternating purine-pyrimidine tracks longer than four nucleotides in length were found in 11% of IRR, though only in 4% of TLD. I discuss the possible biological significance of these findings and present an appendix containing the sequences in the 10 bp vicinity of the non-homologous recombination sites analyzed.