Activity of Synthetic Antimicrobial Peptide GH12 against Oral Streptococci
Activity of Synthetic Antimicrobial Peptide GH12 against Oral Streptococci
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合成抗菌肽GH12对口腔链球菌的活性
DOI：
10.1159/000442898
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发表时间：
2016-01-01
期刊：
影响因子：
4.2
通讯作者：
Zhang, Linglin
中科院分区：
文献类型：
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作者：
Tu, Huanxin;Fan, Yingying;Zhang, Linglin
控制口腔链球菌等致龋微生物的生长是预防和治疗龋齿活跃个体的一种辅助疗法。本文研究了合成的两亲性α -螺旋抗菌肽GH12 （GLLWHLLHHLLH-NH2）对口腔链球菌的体外抑菌活性。圆二色性研究表明，GH12在模拟膜溶剂存在下呈现α -螺旋构象，反相高效液相色谱研究表明GH12在唾液中保持稳定。该肽对口腔链球菌具有抗菌活性，最低抑菌浓度为6.7 ~ 32.0 μ g/ml。GH12浓度比最低杀菌浓度高4倍，可在20分钟内完全杀死口腔链球菌。共聚焦激光扫描显微镜和扫描电镜观察显示，用GH12治疗口腔链球菌可引起细菌活力和形态的明显变化。通过结晶紫染色和3-（4,5-二甲基噻唑-2-基）-2,5-二苯基溴化四唑测定法定量GH12对生物膜形成和成熟生物膜活力的影响。GH12能有效抑制口腔链球菌，尤其是变形链球菌、sobrinus和唾液链球菌生物膜的形成和代谢活性。这些结果表明，GH12在体外对口腔链球菌具有快速而强的抗菌活性，为临床前和临床研究探索其预防和治疗龋齿的潜力打开了大门。(C) 2016 S. Karger AG，巴塞尔
Controlling the growth of cariogenic microorganisms such as oral streptococci is an adjunct therapy for caries-active individuals to prevent and treat caries. Here we investigated the antimicrobial activity of the synthetic amphipathic alpha-helical antimicrobial peptide GH12 (GLLWHLLHHLLH-NH2) against oral streptococci in vitro. Circular dichroism studies showed that GH12 takes on an alpha-helical conformation in the presence of membrane-mimicking solvents, and reversed-phase high-performance liquid chromatography studies showed that GH12 remains stable in saliva. The peptide showed bactericidal activity against oral streptococci, with minimum inhibitory concentrations ranging from 6.7 to 32.0 mu g/ml. GH12 concentrations 4-fold higher than the minimum bactericidal concentration completely killed oral streptococci within 20 min. Treating oral streptococci with GH12 caused noticeable changes in bacterial viability and morphology based on confocal laser scanning microscopy and scanning electron microscopy. Effects of GH12 on biofilm formation and on viability of mature biofilm were quantified by crystal violet staining and the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay. GH12 effectively inhibited biofilm formation and metabolic activity in biofilms of oral streptococci, especially S. mutans, S. sobrinus and S. salivarius. These results suggest that GH12 shows rapid and strong antimicrobial activity against oral streptococci in vitro, opening the door to preclinical and clinical studies to explore its potential for caries prevention and treatment. (C) 2016 S. Karger AG, Basel