Research on the relationship between brain anoxia at different regional oxygen saturations and brain damage using near-infrared spectroscopy
Research on the relationship between brain anoxia at different regional oxygen saturations and brain damage using near-infrared spectroscopy
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近红外光谱研究不同区域氧饱和度下脑缺氧与脑损伤的关系
DOI：
10.1088/0967-3334/28/10/010
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发表时间：
2007-10-01
影响因子：
3.2
通讯作者：
Ding, Haishu
中科院分区：
文献类型：
--
作者：
Hou, Xinlin;Ding, Haiyan;Ding, Haishu
本研究旨在探讨不同脑区域氧饱和度（rSO2）下新生猪的生理参数、脑电图及脑组织形态学的差异，为临床应用rSO2判断脑损伤及损伤程度提供依据。采用无创近红外光谱（NIRS）技术对27头新生猪的rSO2进行了监测。新生猪机械通气并吸入3-11%的氧气30 min后，根据吸入不同浓度的氧气引起的脑内rSO2进行分组。rSO2 < 30%、30-35%、35-40%、40-50%组各6只，rSO2 < 60%组（正常对照）3只。在实验过程中监测生理参数和脑电图。脑损伤后72h脱皮处死，对海马CA1区脑组织进行光镜检查和超微结构病理分析。rSO2 bbb40 %组平均动脉压（MAP）稳定，缺氧后血乳酸、脑电图波幅、光镜及超微结构无明显变化。rSO2在30% ~ 40%之间时，MAP稳定，血乳酸水平升高，代谢性酸中毒发生，脑电图波幅无明显变化，光镜下脑组织出现缺血性改变，海马CA1区神经元线粒体损伤。rSO2 < 30%时，发生循环衰竭，血乳酸水平升高，出现严重代谢性酸中毒，脑电图波幅明显下降，光镜下出现空泡化和细胞碎片，海马CA1区神经元线粒体严重损伤。在不同缺氧程度下，当rSO2在30% ~ 40%之间时，新生猪发生脑损伤，线粒体功能区受损。当rSO2低于30%时，脑功能严重异常，线粒体功能区出现严重形态学损伤是缺氧后脑神经细胞能量代谢紊乱和神经系统后遗症的基础。
The objective of this paper is to investigate the difference in physiological parameters, EEG and morphology of brain tissues in newborn pigs with different regional oxygen saturations of brain (rSO2) and provide a basis for the determination of brain injury and degree of injury with the rSO2 in clinical practice. A noninvasive near-infrared spectroscopy (NIRS) technique was used to monitor the rSO2 of 27 newborn pigs. After mechanical ventilation and inhalation of 3–11% oxygen for 30 min by the newborn pigs, the pigs were grouped according to the rSO2 in the brain caused by inhalation of different concentrations of oxygen. There were six animals each in rSO2 < 30%, 30–35%, 35–40%, 40–50% groups and three animals in the rSO2 > 60% group (normal control). The physiological parameters and the EEG were monitored during the experiment. The animals were sacrificed by decollation at 72 hours after brain injury, and light microscope examination and pathological analysis of the ultrastructure were conducted on the brain tissues in the CA1 zone of hippocampi. In rSO2 > 40% groups, the mean arterial pressure (MAP) was stable and there were no significant changes in blood lactic acid, amplitudes of the EEG, light microscopic findings and ultrastructure after hypoxia. When the rSO2 was between 30% and 40%, the MAP was stable, the level of blood lactic acid increased, metabolic acidosis occurred, there was no significant change in the amplitudes of the EEG, there were ischemic changes in brain tissues under a light microscope and there was an injury of mitochondria in the neurons in the CA1 zone of hippocampi. When the rSO2 was less than 30%, circulatory failure occurred, the level of blood lactic acid increased, there was serious metabolic acidosis, the amplitudes of the EEG significantly decreased, there were vacuolization and broken fragments of cells under the light microscope and the mitochondria in the neurons in the CA1 zone of hippocampi were seriously injured. Under varying degrees of hypoxia, when the rSO2 is between 30% and 40%, brain injury occurs and the functional zones of mitochondria are injured in newborn pigs. When the rSO2 is less than 30%, the brain functions are seriously abnormal, and the serious morphological impairment in the functional zones of mitochondria is the basis for the disturbance of energy metabolism in brain neurocytes after hypoxia and the sequelae of the nervous system.