Type II glucocorticoid receptor immunoreactivity in the mossy cells of the rat and the mouse hippocampus.
Type II glucocorticoid receptor immunoreactivity in the mossy cells of the rat and the mouse hippocampus.
复制标题
大鼠和小鼠海马苔藓细胞中 II 型糖皮质激素受体的免疫反应性。
DOI：
10.1002/hipo.10045
复制
发表时间：
2003
期刊：
影响因子：
--
通讯作者：
Bulloch,Karen
中科院分区：
文献类型：
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作者：
Patel,Anisha;Bulloch,Karen
已知海马主要神经元、颗粒细胞和锥体细胞表达 II 型糖皮质激素受体 (GR)，并且相信糖皮质激素 (GC) 至少通过 GR 介导其部分作用。在严重应激和创伤的情况下，这些主细胞很容易受到损伤，并且GR可能会加剧这种机制。苔藓细胞是一种兴奋性齿状回神经元，在创伤后也会受损，据报道，在红藻氨酸诱导的癫痫发作后，大鼠中超过 50% 的苔藓细胞受损。然而，尚未确定 GC 是否在保护或加剧这种重要海马细胞类型的损伤方面发挥任何作用。在本研究中，我们利用免疫细胞化学双标记技术对大鼠和小鼠的苔藓细胞中 GR 的存在进行了评估。为了鉴定大鼠体内的苔藓细胞，我们使用了谷氨酸受体亚基 2/3 (GluR2/3) 的抗体。除了 GluR2/3 抗体外，在小鼠中还使用了钙结合蛋白钙结合蛋白 (CR) 的抗体来识别苔藓细胞。我们的结果表明，GR 免疫反应性 (IR) 与 GluR2/3-IR 在大约 90% 的大鼠和小鼠苔藓细胞中共定位。此外，在小鼠海马的 CR-IR 苔藓细胞中鉴定出 GR-IR，而大鼠和小鼠的 CR-IR 中间神经元则呈 GR-IR 阴性。苔藓细胞上 GR 的存在可能表明 GC 介导这些细胞的细胞活性的能力。海马 2003；13：59–66。 © 2003 Wiley-Liss, Inc.
Hippocampal principal neurons, granule and pyramidal cells, are known to express type II glucocorticoid receptors (GR) and it is believed that glucocorticoids (GC) mediate at least some of their effects through GR. Under conditions of severe stress and trauma, these principal cells are vulnerable to damage and this mechanism may be exacerbated by GR. The mossy cell, an excitatory dentate gyrus neuron, is also damaged following trauma, with over 50% reported loss in rats after kainate‐induced seizures. However, it has not been determined if GC play any role in protecting or exacerbating damage to this important hippocampal cell type. In the present study, we have undertaken an evaluation of the presence of GR in mossy cells of the rat and mouse utilizing an immunocytochemical double‐labeling technique. To identify mossy cells in the rat, we utilized an antibody to the glutamate receptor subunit 2/3 (GluR2/3). In addition to GluR2/3 antibodies, in the mouse, an antibody to the calcium‐binding protein, calretinin (CR), to identify mossy cells was also employed. Our results show that GR immunoreactivity (IR) was colocalized with GluR2/3‐IR in approximately 90% of the rat and the mouse mossy cells. In addition, GR‐IR was identified in the CR‐IR mossy cells in the mouse hippocampus, whereas the CR‐IR interneurons of rat and mouse were negative for GR‐IR. The presence of GR on mossy cells may indicate the ability of GC to mediate cellular activity of these cells. Hippocampus 2003;13:59–66. © 2003 Wiley‐Liss, Inc.