Synthesis and Characterization of Oxidized Polysaccharides for In Situ Forming Hydrogels
Synthesis and Characterization of Oxidized Polysaccharides for In Situ Forming Hydrogels
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DOI：
10.3390/biom10081185
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发表时间：
2020-08-01
期刊：
影响因子：
5.5
通讯作者：
Groth, Thomas
中科院分区：
文献类型：
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作者：
Muhammad, Muhammad;Willems, Christian;Groth, Thomas
多糖在组织工程中被广泛用作支架和水凝胶的构建块，这可能需要它们的化学修饰以允许交联。本研究的目的是通过氧化海藻酸盐（oALG）和氧化透明质酸（oHA）的醛基与羧甲基壳聚糖（CMC）的氨基之间的亚胺键形成共价反应，建立可用于原位凝胶的氧化海藻酸盐（oALG）和氧化透明质酸（oHA）库。本实验研究了高酸钠浓度和反应时间对卵磷脂醛含量、衍生物分子量和卵磷脂对3T3-L1成纤维细胞毒性的影响。结果表明，所有oPs的分子量均随氧化而降低，氧化程度在oHA中普遍高于oALG。研究表明，只有氧化度在25%以上的oPs才具有细胞毒性。对oPs与CMC交联的初步研究也表明，具有中等细胞毒性的高氧化oPs形成了相当软的凝胶。本研究结果表明，oALG和oHA可作为原位凝胶水凝胶或墨水用于生物打印，并可用于组织工程和控释。
Polysaccharides are widely used as building blocks of scaffolds and hydrogels in tissue engineering, which may require their chemical modification to permit crosslinking. The goal of this study was to generate a library of oxidized alginate (oALG) and oxidized hyaluronic acid (oHA) that can be used for in situ gelling hydrogels by covalent reaction between aldehyde groups of the oxidized polysaccharides (oPS) and amino groups of carboxymethyl chitosan (CMC) through imine bond formation. Here, we studied the effect of sodium periodate concentration and reaction time on aldehyde content, molecular weight of derivatives and cytotoxicity of oPS towards 3T3-L1 fibroblasts. It was found that the molecular weights of all oPs decreased with oxidation and that the degree of oxidation was generally higher in oHA than in oALG. Studies showed that only oPs with an oxidation degree above 25% were cytotoxic. Initial studies were also done on the crosslinking of oPs with CMC showing with rheometry that rather soft gels were formed from higher oxidized oPs possessing a moderate cytotoxicity. The results of this study indicate the potential of oALG and oHA for use as in situ gelling hydrogels or inks in bioprinting for application in tissue engineering and controlled release.