High polymorphism in Plasmodium vivax merozoite surface protein-5 (MSP5)
High polymorphism in Plasmodium vivax merozoite surface protein-5 (MSP5)
复制标题
DOI：
10.1017/s0031182006001168
复制
发表时间：
2006-12-01
期刊：
影响因子：
2.4
通讯作者：
Patarroyo, M. A.
中科院分区：
文献类型：
--
作者：
Gomez, A.;Suarez, C. F.;Patarroyo, M. A.
研究间日疟原虫表面蛋白的遗传变异是研制多组分抗疟疾疫苗的关键。本研究的目的是扩增、克隆和测序间日疟原虫裂殖子表面蛋白5(PvMSP5)基因，该基因来自哥伦比亚不同疟疾传播率地区的感染者。与外显子I相比，内含子和外显子II(包括GPI锚定和EGF样结构域)都高度保守；外显子I变异最大，大部分重组事件发生在它内部。没有观察到地理分组。Nei-Gojobori检验显示，在这里分析的样本中有显著的正选择，而Tajima和Fu和Li测试则呈现中性选择模式。这些结果反映了PvMSP5等位基因之间的局部变异模式，以及功能和免疫压力，其中作用于外显子I的选择性力量可能比作用于外显子II的选择性更强，提示后者可能是抗疟疾疫苗中的一个重要区域。
A key issue relating to developing multi-component anti-malarial vaccines, lies in studying Plasmodium vivax surface proteins' genetic variation. The present work was aimed at amplifying, cloning and sequencing the gene encoding P. vivax merozoite surface protein 5 (PvMSP5) in samples obtained from infected patients from Colombian areas having varying malaria transmission rates.dagger Our results have revealed that PvMSP5 is one of the P. vivax surface proteins having greater polymorphism, this being restricted to specific protein regions. The intron and exon II (which includes the GPI anchor and EGF-like domain) were both highly conserved when compared to exon I; exon I displayed the greatest variation and most of the recombination events occurred within it. No geographical grouping was observed. The Nei-Gojobori test revealed significant positive selection in the samples analysed here, whereas Tajima and Fu and Li tests presented a neutral selection pattern. The results reflected a localized variation pattern, recombination between PvMSP5 alleles and also functional and immune pressures, where stronger selective forces might be acting on exon I than on exon II, suggesting that the latter could be an important region to be included in an anti-malarial vaccine.