Simultaneous quantification of twelve bioactive components in San-Huang-Xie-Xin-Tang by HPLC
Simultaneous quantification of twelve bioactive components in San-Huang-Xie-Xin-Tang by HPLC
复制标题
DOI：
10.1002/pca.947
复制
发表时间：
2006-11-01
影响因子：
3.3
通讯作者：
Tsai, Yi-Hung
中科院分区：
文献类型：
--
作者：
Huang, Yaw-Bin;Wu, Pao-Chu;Tsai, Yi-Hung
建立了一种简便、可靠的高效液相色谱-紫外检测法（280 nm），用于三黄泻心汤（SHXX 7）中12种活性成分的同时分析。一个相对简单的提取程序，采用和优化，并在1小时内获得分离的成分，使用反相柱梯度洗脱下与乙腈和缓冲液含有0.01 M的1-戊磺酸钠（pH 3）。分析物的检测下限范围为25 - 75 ng/mL。与各校准曲线相关的相关系数均大于0.99。该方法的精密度和准确度范围为：低浓度水平为1.0 - 10.5%，中等水平为0.8 - 8.7%，高水平为1.2 - 5.8%。在SHXXT的商业产品中，黄芩苷和小檗碱的含量最高，在一个样品中分别高达4.0%和3.3%。该方法能快速、有效地分析含黄芪、麦冬、丹参的中草药及中药制剂中的成分。版权所有（c）2006约翰威利父子有限公司。
A simple and reliable high-performance liquid chromatographic method with ultraviolet detection (280 run) has been developed for the simultaneous analysis of 12 bioactive components in San-huang-xie-xin-tang (SHXX7), a traditional Chinese medicine containing Rhei rhizome, Coptidis rhizome and Scutellariae radix. A relatively simple extraction procedure was employed and optimised, and separation of the components was obtained within 1 h using a reversed-phase column under gradient elution with acetenitrile and a buffer containing 0.01 M sodium 1-pentanesulphonate (pH 3). The lower limit of detection for the analytes ranged from 25 to 75 ng/mL. The correlation coefficients associated with each calibration curve were greater than 0.99. The precision and accuracy of the method ranged from 1.0 to 10.5% at low concentration levels, 0.8 to 8.7% at medium levels and 1.2 to 5.8% at high levels. In commercial products of SHXXT, baicalin and berberine were present in the highest amounts with levels up to 4.0 and 3.3%, respectively, in one sample. The HPLC method was able rapidly and efficiently to analyse constituents in crude herb and traditional Chinese medicinal preparations containing Rhei rhizome, Coptidis rhizome and Scutellariae radix. Copyright (c) 2006 John Wiley & Sons, Ltd.