Proteome differences associated with fat accumulation in bovine subcutaneous adipose tissues
Proteome differences associated with fat accumulation in bovine subcutaneous adipose tissues
复制标题
与牛皮下脂肪组织脂肪积累相关的蛋白质组差异
作者：
Yong Mei Zhao;U. Basu;M. Dodson;John A Basarb;L. Guan
背景由于人类过量摄入脂肪对健康的影响，研究人员对红肉产品的脂肪成分很感兴趣。我们假设蛋白质表达的差异对肉牛发育和生长过程中脂肪组织的形成有影响。因此，在本研究中，我们评估了 35 头不同背脂 (BF) 厚度的杂交肉牛 [夏洛莱 × 红安格斯 (CHAR) (n = 13) 和赫里福德 × 安格斯 (HEAN) (n = 22)] 皮下脂肪组织可辨别蛋白质组的差异。目的是鉴定与肉牛脂肪组织形成相关的特定蛋白质标记。结果在每个杂交组中检测到并比较了大约 541-580 个蛋白质点，其中 33 个和 36 个蛋白质点分别在 HEAN 和 CHAR 杂交床的高 BF 厚度和低 BF 厚度的组织之间表现出表达差异。膜联蛋白 1 蛋白在 BF 厚度较高的两种杂交公牛中高度表达 (p < 0.05)，并且通过蛋白质印迹分析进一步验证了这一点。在 CHAR 动物的 13 个组织和 HEAN 动物的 22 个组织中，膜联蛋白 1 的相对表达量在高 BF 厚度和低 BF 厚度的组织之间存在显着差异（p < 0.05）。 结论 在两种杂交公牛中，膜联蛋白 1 蛋白表达的增加与较高的 BF 厚度相关。该结果为未来研究开发用于评估不同 BF 厚度的动物的蛋白质标记物奠定了基础。
BackgroundThe fat components of red meat products have been of interest to researchers due to the health aspects of excess fat consumption by humans. We hypothesized that differences in protein expression have an impact on adipose tissue formation during beef cattle development and growth. Therefore, in this study we evaluated the differences in the discernable proteome of subcutaneous adipose tissues of 35 beef crossbred steers [Charolais × Red Angus (CHAR) (n = 13) and Hereford × Angus (HEAN) (n = 22)] with different back fat (BF) thicknesses. The goal was to identify specific protein markers that could be associated with adipose tissue formation in beef cows.ResultsApproximately 541-580 protein spots were detected and compared in each crossbred group, and 33 and 36 protein spots showed expression differences between tissues with high and low BF thicknesses from HEAN and CHAR crossbed, respectively. The annexin 1 protein was highly expressed in both crossbred steers that had a higher BF thickness (p < 0.05) and this was further validated by a western blot analysis. In 13 tissues of CHAR animals and 22 tissues of HEAN animals, the relative expression of annexin 1 was significantly different (p < 0.05) between tissues with high and low BF thicknesses.ConclusionThe increased expression of annexin 1 protein has been found to be associated with higher BF thickness in both crossbred steers. This result lays the foundation for future studies to develop the protein marker for assessing animals with different BF thickness.
影响因子：
3.5
作者：
Burton, GR;Nagarajan, R;McGehee, RE
通讯作者：
McGehee, RE
影响因子：
10.5
作者：
E. Rosen;C. Walkey;P. Puigserver;B. Spiegelman
通讯作者：
E. Rosen;C. Walkey;P. Puigserver;B. Spiegelman
生长激素通过主要生长激素受体基因启动子的信号转导器和转录激活剂5的激活来刺激牛生长激素受体信使核糖核酸的肝脏表达。
影响因子：
4.8
作者：
Jiang,Honglin;Wang,Ying;Wu,Miaozong;Gu,Zhiliang;Frank,StuartJ;Torres-Diaz,Roberto
通讯作者：
Torres-Diaz,Roberto