Genome-Centered Metagenomics Analysis Reveals the Symbiotic Organisms Possessing Ability to Cross-Feed with Anammox Bacteria in Anammox Consortia.
Genome-Centered Metagenomics Analysis Reveals the Symbiotic Organisms Possessing Ability to Cross-Feed with Anammox Bacteria in Anammox Consortia.
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DOI：
10.1021/acs.est.8b02599
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发表时间：
2018-09
影响因子：
11.4
通讯作者：
Yunpeng Zhao;Shufeng Liu;Bo Jiang;Ying Feng;Tingting Zhu;H. Tao;Xi Tang;Sitong Liu
中科院分区：
文献类型：
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作者：
Yunpeng Zhao;Shufeng Liu;Bo Jiang;Ying Feng;Tingting Zhu;H. Tao;Xi Tang;Sitong Liu
利用厌氧氨氧化菌群高效处理污水已引起人们的广泛关注，但由于厌氧氨氧化菌的纯源难以获得，共生菌在厌氧氨氧化中的潜在作用还不明确。在这里，我们结合了长期的反应器运行，基因组为中心的宏基因组学，社区功能结构，和代谢途径重建，以揭示多个潜在的交叉喂养在厌氧氨氧化反应器启动期间，根据37个回收的宏基因组组装的基因组（MAG）。我们发现Armatimonadetes和Proteobacteria可以为厌氧氨氧化细菌提供次级代谢产物异蝶呤辅因子和叶酸，以利于它们的活性和生长。根据厌氧氨氧化菌产生的部分核苷酸糖，研究了氯弯曲菌的胞外多糖合成功能。叶绿素相关细菌具有将厌氧氨氧化菌产生的胞外蛋白降解为氨基酸的能力，从而影响群体聚集。此外，Chloroflexi附属细菌含有亚硝酸盐循环的基因，并可能在反应器启动期间在厌氧氨氧化性能中发挥双重作用。厌氧氨氧化菌群落中的交叉投喂为理解微生物间的相互作用提供了一个新的视角，并强调了共生菌在厌氧氨氧化污水处理工艺中的重要性。
Although using anammox communities for efficient wastewater treatment has attracted much attention, the pure anammox bacteria are difficult to obtain, and the potential roles of symbiotic bacteria in anammox performance are still elusive. Here, we combined long-term reactor operation, genome-centered metagenomics, community functional structure, and metabolic pathway reconstruction to reveal multiple potential cross-feedings during anammox reactor start-up according to the 37 recovered metagenome-assembled genomes (MAGs). We found Armatimonadetes and Proteobacteria could contribute the secondary metabolites molybdopterin cofactor and folate for anammox bacteria to benefit their activity and growth. Chloroflexi-affiliated bacteria encoded the function of biosynthesizing exopolysaccharides for anammox consortium aggregation, based on the partial nucleotide sugars produced by anammox bacteria. Chlorobi-affiliated bacteria had the ability to degrade extracellular proteins produced by anammox bacteria to amino acids to affect consortium aggregation. Additionally, the Chloroflexi-affiliated bacteria harbored genes for a nitrite loop and could have a dual role in anammox performance during reactor start-up. Cross-feeding in anammox community adds a different dimension for understanding microbial interactions and emphasizes the importance of symbiotic bacteria in the anammox process for wastewater treatment.