Site-specific modification of enzymatically synthesized RNA: Transcription initiation and Diels-Alder reaction
Site-specific modification of enzymatically synthesized RNA: Transcription initiation and Diels-Alder reaction
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DOI：
10.1016/s0040-4039(97)10151-4
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发表时间：
1997-11-03
影响因子：
1.8
通讯作者：
Jaschke, A
中科院分区：
文献类型：
--
作者：
Seelig, B;Jaschke, A
由聚乙烯甘氨酸（PEG）接头由蒽环系与5'-磷酸偶联的蒽环系系统组成的起始核苷酸被化学合成。当添加到转录反应中时，这些启动器被特异性地纳入RNA转录物中，从而通过柔性接头选择性地连接到5'-End的RNA偶联物中。除了敏感的荧光检测外，蒽还允许通过Diels-Alder反应对马来酰亚胺进行方便的修饰。 （c）1997 Elsevier Science Ltd.
Initiator nucleotides consisting of an anthracene ring system coupled to the 5'-phosphate of guanosine monophosphate via a polyethylene glycol (PEG) linker were chemically synthesized. When added to transcription reactions, these initiators were site-specifically incorporated into RNA transcripts yielding RNA conjugates with a fluorophore selectively attached to the 5'-end via a flexible linker. Besides sensitive fluorescence detection, the anthracene allowed the convenient modification with maleimides by Diels-Alder reaction. (C) 1997 Elsevier Science Ltd.