Discovery of HLA-E-Presented Epitopes: MHC-E/Peptide Binding and T-Cell Recognition.
Discovery of HLA-E-Presented Epitopes: MHC-E/Peptide Binding and T-Cell Recognition.
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DOI：
10.1007/978-1-0716-2712-9_2
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发表时间：
2022
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了解肽、HLA-E分子和TCR之间免疫突触的相互作用对于有效靶向人类保护性HLA-E限制性t细胞反应至关重要。在这里，我们描述了基于生成MHC-E/肽复合物（MHC-E一般包括人类和非人类物种中的hla样分子，而HLA-E专门指人类分子）的三种技术，这些技术允许通过结合试验和使用肽负载HLA-E四聚体在分子水平上研究MHC-E/肽结合，以检测、分离和研究肽特异性HLA-E限制性人t细胞。
Understanding the interactions involved during the immunological synapse between peptide, HLA-E molecules, and TCR is crucial to effectively target protective HLA-E-restricted T-cell responses in humans. Here we describe three techniques based on the generation of MHC-E/peptide complexes (MHC-E generically includes HLA-E-like molecules in human and nonhuman species, while HLA-E specifically refers to human molecules), which allow to investigate MHC-E/peptide binding at the molecular level through binding assays and by using peptide loaded HLA-E tetramers, to detect, isolate, and study peptide-specific HLA-E-restricted human T-cells.