Age-related changes in fatty acid profile and locomotor activity rhythms in Nothobranchius korthausae
Age-related changes in fatty acid profile and locomotor activity rhythms in Nothobranchius korthausae
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DOI：
10.1016/j.exger.2011.08.009
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发表时间：
2011-12-01
影响因子：
3.9
通讯作者：
Mendiola, P.
中科院分区：
文献类型：
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作者：
Lucas-Sanchez, A.;Almaida-Pagan, P. F.;Mendiola, P.
我们的实验室对 Nothobranchius korthausae（一种鲤鱼目鱼类）的生命周期进行了研究，以表征衰老过程。描述了一些形态变化，例如脊柱弯曲、肤色、鳍和眼睛的外观。生长和存活曲线反映了快速的生命周期，初始生长迅速，直到 4 周龄，此后鱼生长速度减慢，在第 40 周达到最终大小。衰老开始于第 48 周，产卵大小和活力下降，动物外观普遍下降（体重和颜色损失、尾鳍退化和白内障发生）。脂肪酸组成随着年龄的增长而变化，成年阶段的不饱和度很高，这反映在高过氧化指数上，如果活性氧（ROS）产生升高，这种情况与氧化损伤的高度敏感性有关。衰老鱼的单不饱和脂肪酸比例增加，过氧化指数较低（成年鱼为 226.5 +/- 19.7，衰老鱼为 120.2 +/- 19.1，P
The life cycle of Nothobranchius korthausae, a Cyprinodontiformes fish, was studied in our laboratory to characterise the ageing process. Some morphological changes, such as spine curvature, skin colour, and fin and eye appearance are described. Growth and survival curves reflected a fast life cycle with rapid initial growth until 4 weeks of age, after which the fish grew more slowly before reaching their final size in week 40. Senescence onset was established at week 48 with a decrease in spawn size and viability and a general decline in the animal's appearance (weight and colouration losses, caudal fin degradation, and cataractogenesis). The fatty acid composition changed with age, with high unsaturation in the adult stage as reflected by a high peroxidation index, a condition that is associated with high susceptibility to oxidative damage if elevated reactive oxygen species (ROS) production occurs. Senescent fish had an increase in monounsaturated fatty acid proportions and a lower peroxidation index (226.5 +/- 19.7 in adults versus 120.2 +/- 19.1 in senescent fish, P