STRUCTURE AND EXPRESSION OF THE MURINE RETINOBLASTOMA GENE AND CHARACTERIZATION OF ITS ENCODED PROTEIN
STRUCTURE AND EXPRESSION OF THE MURINE RETINOBLASTOMA GENE AND CHARACTERIZATION OF ITS ENCODED PROTEIN
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DOI：
10.1073/pnas.86.17.6474
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发表时间：
1989-09-01
影响因子：
11.1
通讯作者：
WEINBERG, RA
中科院分区：
文献类型：
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作者：
BERNARDS, R;SCHACKLEFORD, GM;WEINBERG, RA
我们已经分离出了人视网膜母细胞瘤（Rb）易感基因的小鼠同源物的cDNA克隆。DNA序列分析表明，在编码区和非编码区与人Rb序列高度保守。小鼠Rb蛋白的预测氨基酸序列显示91%的同一性，人的蛋白质。发现这两种蛋白质都含有一个肽序列，让人联想到一个亮氨酸重复基序（“亮氨酸拉链”），也发现在myc，fos和jun癌基因。针对小鼠Rb蛋白的一部分的合成肽抗血清检测用小鼠Rb基因表达构建体瞬时转染的细胞中104-110 kDa的三种蛋白。在小鼠胚胎中，Rb mRNA的表达是普遍存在的，在妊娠13天左右观察到最大表达。在胚胎中，在肝脏和脑中观察到最高水平的表达。与此相反，Rb基因被认为是在一个非常低的水平，在成年小鼠的肝脏被发现在肺，胸腺和脾脏高水平的表达。在小鼠试验中检测到较短的Rb转录本。
We have isolated a cDNA clone of the murine homologue of the human retinoblastoma (Rb) susceptibility gene. DNA sequence analysis reveals a high degree of conservation with the human Rb sequence, both in the coding and in the noncoding regions. The predicted amino acid sequence of the mouse Rb protein shows 91% identity to that of the human protein. Both proteins were found to contain a peptide sequence reminiscent of a leucine-repeat motif ("leucine-zipper") that is also found in the myc, fos, and jun oncogenes. Synthetic peptide antiserum directed against a protion of the mouse Rb protein detects three proteins of 104-110 kDa in cells that were transiently transfected with a mouse Rb gene expression construct. in the mouse embryo the expression of Rb mRNA was ubiquitous, with maximal expression being observed around 13 days of gestation. In the embryo, the ihighest level of expression was observed in liver and brain. In contrast, the Rb gene was found to be expressed at a very low level in adult mouse liver with high levels being found in lung, thymus, and spleen. A shorter Rb transcript was detected in mouse tests.