Effect of Salinity Change on Biomass and Biochemical Composition of Nannochloropsis oculata
Effect of Salinity Change on Biomass and Biochemical Composition of Nannochloropsis oculata
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DOI：
10.1111/j.1749-7345.2011.00538.x
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发表时间：
2012-02-01
影响因子：
2.8
通讯作者：
Huang, Liangmin
中科院分区：
文献类型：
--
作者：
Gu, Na;Lin, Qiang;Huang, Liangmin
盐度波动是影响室外微藻培养的重要因素。研究了盐度变化（Tr-1：3515 g/L，Tr-2：3525 g/L，Tr-3：3535 g/L，Tr-4：3545 g/L，Tr-5：3555 g/L）对微拟球藻（Nannochloropsis oculata）生长和生化组成的影响。结果表明，随着盐度的降低，藻类的吸光度和干生物量增加。随着盐度的增加，藻类的比生长速率（SGR）显著下降。盐度胁迫对藻体色素、叶绿素a和类胡萝卜素含量也有影响，在45 ~ 55 g/L范围内，随着盐度的升高，藻体色素、叶绿素a和类胡萝卜素含量均降低。脂肪酸甲酯（FAME）含量（干生物量的%）随着盐度的增加而增加（例如，Tr-4和Tr-5）。藻类富含C16：0（棕榈酸）、C16：1 n-7（棕榈油酸）和C20：5 n-3（二十碳五烯酸），C16：0含量随着盐度从35 g/L降低到15 g/L而增加，但C16：1 n-7含量在所有处理中都很高，从Tr-1的25.25 +/- 1.42%到Tr-5的27.05 +/- 1.13%。
Salinity fluctuation is an important factor affecting outdoor microalgae culture. This investigation examined the effect of salinity change (Tr-1:3515 g/L, Tr-2:3525 g/L, Tr-3:3535 g/L, Tr-4:3545 g/L, and Tr-5:3555 g/L) on growth and the biochemical composition of Nannochloropsis oculata, a candidate for biodiesel production in indoor photo-bioreactors. Results showed that the algae increased in absorbency and dry biomass as salinity decreased. When the salinity increased, the specific growth rate (SGR) of the algae decreased significantly. The salinity stress also affected the pigments of the algae, the chlorophyll-a, and carotenoid contents of the algae which decreased with the increase of salinity from 45 to 55 g/L. The fatty acid methyl esters (FAME) content (% of dry biomass) increased with the increase of salinity (e.g., Tr-4 and Tr-5). The algae was rich in C16:0 (palmitic acid), C16:1n-7 (palmitoleic acid), and C20:5n-3 (eicosapentaenoic acid), and C16:0 content increased with decreasing salinity from 35 to 15 g/L, but C16:1n-7 content was high in all the treatments ranging from 25.25 +/- 1.42% in Tr-1 to 27.05 +/- 1.13% in Tr-5.