Formation of Protein Disulfide Bonds Catalyzed by OsPDIL1;1 is Mediated by MicroRNA5144-3p in Rice
Formation of Protein Disulfide Bonds Catalyzed by OsPDIL1;1 is Mediated by MicroRNA5144-3p in Rice
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水稻中 OsPDIL1;1 催化蛋白质二硫键的形成由 MicroRNA5144-3p 介导
DOI：
10.1093/pcp/pcx189
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发表时间：
2018
影响因子：
4.9
通讯作者：
Zhang Mingyong
中科院分区：
文献类型：
--
作者：
Xia Kuaifei;Zeng Xuan;Jiao Zhengli;Li Maolin;Xu Weijuan;Nong Qu;ong;Mo Hui;Cheng Taihui;Zhang Mingyong
蛋白质在内质网中的正确折叠对于它们在应激下的稳定性和功能是重要的。蛋白质二硫键异构酶（PDI）OsPDIL 1;1是水稻蛋白质折叠的关键催化剂。在此，microRNA 5144（osa-miR 5144 - 3 p）被报道分别在发育期间和非生物胁迫条件下通过靶向水稻种子和幼苗中的OsPDIL 1;1 mRNA介导蛋白质二硫键的形成。对转基因水稻的表达分析和切割位点的鉴定表明OsPDIL 1;1 mRNA是osa-miR 5144 - 3 p的靶基因。osa-miR 5144 - 3 p和OsPDIL 1;1的表达在发育器官和非生物胁迫下被显示为负调控。与野生型相比，在转基因水稻中下调osa-miR 5144 - 3 p或过表达OsPDIL 1;1显示总蛋白质-二硫键含量增加。这表明蛋白质-二硫键形成通过下调osa-miR 5144 - 3 p或过表达OsPDIL 1而增强;1.与野生型相比，这些转基因水稻植株还表现出对盐和汞胁迫的强抗性。相反，过量表达osa-miR 5144 - 3 p或下调OsPDIL 1;1的转基因水稻植株蛋白质-二硫键含量较低，易受非生物胁迫的影响，籽粒异常，淀粉粒小而松散。这些结果表明OsPDIL 1;1催化的蛋白质-二硫键形成在水稻发育过程中受到osa-miR 5144 - 3 p的调控，并参与水稻对非生物胁迫的抗性。
Correct folding of proteins in the endoplasmic reticulum is important for their stability and function under stress. The protein disulfide isomerase (PDI) OsPDIL1;1 is a key protein-folding catalyst in rice (Oryza sativa L.). Here, microRNA5144 (osa-miR5144-3p) is reported to mediate the formation of protein disulfide bonds via targeting OsPDIL1;1 mRNA in rice seeds and seedlings during development and under conditions of abiotic stress, respectively. Expression analysis of transgenic rice and identification of cleavage sites showed that OsPDIL1;1 mRNA is a target of osa-miR5144-3p. Expression of osa-miR5144-3p and OsPDIL1;1 was shown to be inversely regulated in developing organs and under abiotic stress. The down-regulation of osa-miR5144-3p or overexpression of OsPDIL1;1 in transgenic rice showed increased total protein-disulfide bond content, compared with the wild type. This indicates that protein-disulfide bond formation is enhanced by down-regulation of osa-miR5144-3p or overexpression of OsPDIL1;1. These transgenic rice plants also displayed strong resistance to salinity and mercury stress, in comparison with the wild type. In contrast, the transgenic rice plants overexpressing osa-miR5144-3p or down-regulating OsPDIL1;1 had a lower protein-disulfide bond content; they were susceptible to abiotic stress and produced abnormal grains with small and loosely packed starch granules. These results indicate that protein-disulfide bond formation catalyzed by OsPDIL1;1 is modulated by osa-miR5144-3p in rice during development and is involved in resistance to abiotic stress.