CHRONIC ENCEPHALITIS AND EPILEPSY (RASMUSSEN ENCEPHALITIS) - DETECTION OF CYTOMEGALOVIRUS AND HERPES-SIMPLEX VIRUS-1 BY THE POLYMERASE CHAIN-REACTION AND IN-SITU HYBRIDIZATION
CHRONIC ENCEPHALITIS AND EPILEPSY (RASMUSSEN ENCEPHALITIS) - DETECTION OF CYTOMEGALOVIRUS AND HERPES-SIMPLEX VIRUS-1 BY THE POLYMERASE CHAIN-REACTION AND IN-SITU HYBRIDIZATION
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DOI：
10.1212/wnl.45.1.108
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发表时间：
1995-01-01
期刊：
影响因子：
9.9
通讯作者：
ZIELENSKA, M
中科院分区：
文献类型：
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作者：
JAY, V;BECKER, LE;ZIELENSKA, M
我们对10例顽固性癫痫患者的手术切除或尸检材料进行了慢性脑炎的病理诊断，并对标本进行了单纯疱疹病毒（HSV）I和2和巨细胞病毒（CMV）的免疫组织化学研究，以及对病毒DNA序列（HSV 1，HSV 2和CMV）的聚合酶链反应（PCR）。我们还评估了8例病理记录或临床疑似脑炎的患者（非癫痫）和5例无脑炎的癫痫患者的切除术。所有病例的病毒抗原免疫组化均为阴性。PCR检测10例慢性脑炎癫痫患者中6例CMV阳性，2例HSV 1阳性。我们证明CMV原位杂交在两个PCR阳性的6例CMV。我们没有发现任何病毒序列的PCR在五个癫痫无脑炎。8例临床怀疑或病理证实的脑炎患者（非癫痫）中，2例PCR显示CMV序列。这些观察结果表明，PCR允许检测病毒序列在某些情况下，慢性脑炎与癫痫，可能会错过原位杂交。
We made a pathologic diagnosis of chronic encephalitis on surgical resections or autopsy material in 10 patients with intractable seizures and studied the specimens by immunohistochemistry for herpes simplex virus (HSV) I and 2 and cytomegalovirus (CMV) as well as by the polymerase chain reaction (PCR) for Viral DNA sequences (HSV1, HSV2, and CMV). We also assessed eight patients (nonepileptic) with pathologically documented or clinically suspected encephalitis and five resections from epileptics without encephalitis. Immunohistochemistry for viral antigens was negative in all cases. Using PCR assay, CMV was present in six and HSV1 in two of 10 epilepsy patients with chronic encephalitis. We demonstrated CMV by in situ hybridization in two of the six patients positive for CMV by PCR. We found no viral sequences by PCR in five epileptics without encephalitis. Of the eight patients (nonepileptic) with clinically suspected or pathologically confirmed encephalitis, two cases showed CMV sequences by PCR. These observations suggest that PCR allows detection of viral sequences in some cases of chronic encephalitis associated with epilepsy that may be missed by in situ hybridization.