The cluA- mutant of Dictyostelium identifies a novel class of proteins required for dispersion of mitochondria.
The cluA- mutant of Dictyostelium identifies a novel class of proteins required for dispersion of mitochondria.
复制标题
盘基网柄菌的 cluA 突变体鉴定了线粒体分散所需的一类新型蛋白质。
DOI：
10.1073/pnas.94.14.7308
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发表时间：
1997
影响因子：
11.1
通讯作者：
Clarke,M
中科院分区：
文献类型：
--
作者：
Zhu,Q;Hulen,D;Liu,T;Clarke,M
盘基网眼菌的luA基因编码一个新的150 kDa的蛋白。簇A的破坏导致线粒体在细胞中心附近聚集。这是与正常细胞的显著不同，正常细胞的线粒体均匀分布在细胞质中。突变细胞群体还表现出多核细胞的频率增加，这表明细胞质分裂受损。两种表型都可以通过用携带组成性表达的CluA基因的质粒转化CluA−细胞来逆转。该基因产物的预测序列与酿酒酵母和秀丽线虫基因组中由开放阅读框编码的序列同源，但与任何已知蛋白质不同源。唯一的例外是一个与Kinesin轻链中的42个残基有一些同源性的短区域，它可能在蛋白质相互作用中发挥作用。这些研究确定了一类新的蛋白质，似乎是适当分布线粒体所必需的。
ThecluAgene ofDictyostelium discoideumencodes a novel 150-kDa protein. Disruption ofcluAresults in clustering of mitochondria near the cell center. This is a striking difference from normal cells, whose mitochondria are dispersed uniformly throughout the cytoplasm. The mutant cell populations also exhibit an increased frequency of multinucleated cells, suggesting an impairment in cytokinesis. Both phenotypes are reversed by transformation ofcluA−cells with a plasmid carrying a constitutively expressedcluAgene. The predicted sequence of thecluAgene product is homologous to sequences encoded by open reading frames in the genomes ofSaccharomyces cerevisiaeandCaenorhabditis elegans, but not to any known protein. The only exception is a short region with some homology to the 42-residue imperfect repeats present in the kinesin light chain, which probably function in protein–protein interaction. These studies identify a new class of proteins that appear to be required for the proper distribution of mitochondria.