PGRL1 overexpression in Phaeodactylum tricornutum inhibits growth and reduces apparent PSII activity.
PGRL1 overexpression in Phaeodactylum tricornutum inhibits growth and reduces apparent PSII activity.
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DOI：
10.1111/tpj.14872
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发表时间：
2020-06
期刊：
影响因子：
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通讯作者：
Lu Zhou;Shan Gao;Songcui Wu;Danxiang Han;Hui Wang;Wenhui Gu;Q. Hu;Jing Wang;Guangce Wang
中科院分区：
文献类型：
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作者：
Lu Zhou;Shan Gao;Songcui Wu;Danxiang Han;Hui Wang;Wenhui Gu;Q. Hu;Jing Wang;Guangce Wang
依赖于PGRL1的光系统I(PSI)周围的循环电子传递(CET)在响应包括强光在内的不同胁迫中发挥着重要作用。尽管PGRL1在高等植物和绿藻中的功能已经被深入研究，但关于PGRL1在硅藻中的分子机制研究很少。我们建立了硅藻模式种三角褐指藻的PGRL1过表达和基因敲除转化子，并研究了在恒定和波动的光照条件下对生长和光合作用的影响。PGRL1的过度积累导致生长速率和表观光系统II(PSII)活性显著下降，并导致强光下表观PSII活性的相反变化，对光合作用的影响类似于PSII抑制剂。我们的结果表明，过表达PGRL1可以降低PSII的表观效率，并抑制三角根霉的生长。这些发现为PGRL1的积累主要围绕PSII而不是PSI发挥作用提供了生理学证据。
PGRL1-dependent cyclic electron transport (CET) around photosystem I (PSI) plays important roles in response to different stresses, including high light. Although the function of PGRL1 in higher plants and green algae have been thoroughly investigated, little information is available on the molecular mechanism of PGRL1 in diatoms. We created PGRL1 overexpression and knockdown transformants of Phaeodactylum tricornutum, the diatom model species, and investigated the impact on growth and photosynthesis under constant and fluctuating light conditions. PGRL1 over-accumulation resulted in significant decreases in growth rate and apparent photosystem II (PSII) activity and led to an opposing change of apparent PSII activity when turning to high light, demonstrating a similar influence on photosynthesis as a PSII inhibitor. Our results suggested that PGRL1 overexpression can reduce apparent efficiency of PSII and inhibit growth in P. tricornutum. These findings provide physiological evidence that accumulation of PGRL1 mainly functions around PSII instead of PSI.