Electrotransfer into skeletal muscle for protein expression.
Electrotransfer into skeletal muscle for protein expression.
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DOI：
10.2174/156652306778520656
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发表时间：
2006-09
影响因子：
3.6
通讯作者：
Capucine Trollet;C. Bloquel;Daniel Scherman;P. Bigey
中科院分区：
文献类型：
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作者：
Capucine Trollet;C. Bloquel;Daniel Scherman;P. Bigey
一种高效、安全的在体内传递DNA的方法是多种目的的需要，如基因功能研究和基因治疗应用。在目前正在研究的不同的非病毒传递方法中，体内DNA电转移已被证明是最有效和最简单的方法之一。该技术是一种基因传递的物理方法，包括在DNA注射后局部应用电脉冲。虽然这项技术可以应用于活体动物的几乎任何组织，包括肿瘤、皮肤、肝脏、肾脏、动脉、视网膜、角膜甚至大脑，但本文将重点介绍质粒DNA电转移到骨骼肌及其在基因治疗、疫苗接种或功能研究中的可能应用。骨骼肌是DNA电转移的一个很好的目标，因为它体积大，易于获取，是一个能够表达几种局部和全身因子的内分泌器官，肌肉纤维作为有丝分裂后的细胞具有很长的寿命，允许长期的基因表达。在这篇综述中，我们描述了DNA电转移的机制，评估了与该技术相关的毒性和安全性，并重点介绍了近年来在动物模型中证明的电转移的重要治疗应用。
An efficient and safe method to deliver DNA in vivo is a requirement for several purposes, such as study of gene function and gene therapy applications. Among the different non-viral delivery methods currently under investigation, in vivo DNA electrotransfer has proven to be one of the most efficient and simple. This technique is a physical method of gene delivery consisting in local application of electric pulses after DNA injection. Although this technique can be applied to almost any tissue of a living animal, including tumors, skin, liver, kidney, artery, retina, cornea or even brain, this review will focus on electrotransfer of plasmid DNA into skeletal muscle and its possible uses in gene therapy, vaccination, or functional studies. Skeletal muscle is a good target for electrotransfer of DNA as it is: a large volume easily accessible, an endocrine organ capable of expressing several local and systemic factors, and muscle fibres as post-mitotic cells have a long lifespan that allows long-term gene expression. In this review, we describe the mechanism of DNA electrotransfer, we assess toxicity and safety considerations related to this technique, and we focus on important therapeutic applications of electrotransfer demonstrated in animal models in recent years.