The full genome sequence of three strains of Jamestown Canyon virus and their pathogenesis in mice or monkeys.
The full genome sequence of three strains of Jamestown Canyon virus and their pathogenesis in mice or monkeys.
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DOI：
10.1186/1743-422x-8-136
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发表时间：
2011-03-24
期刊：
影响因子：
4.8
通讯作者：
Whitehead SS
中科院分区：
文献类型：
--
作者：
Bennett RS;Nelson JT;Gresko AK;Murphy BR;Whitehead SS
詹姆斯敦峡谷病毒（Jamestown Canyon virus，JCV），布尼亚病毒科，是一种蚊媒病原体，在美国和加拿大流行，可引起人类脑炎，被认为是对公共卫生的新威胁。该病毒在基因上与欧洲流行的Inkoo病毒相似，表明北方大部分地区含有JCV或类似变体。我们已经完成了三个分离株的JCV收集在地理上不同的位置超过57年的时间跨度的序列。S、M和L片段的三种菌株的核苷酸同一性分别为90、83和85%，而N、NSS、M聚、GN、NSM、GC和L蛋白的预测氨基酸序列的同一性百分比分别为97、91、94、98、91、94和97%。在瑞士韦伯斯特小鼠中，每种JCV分离株均表现出低神经侵袭性但高感染性。三个JCV分离物中的两个在IC接种后具有高度神经毒力，而一个分离物JCV/03/CT表现出低神经毒力。在恒河猴中，JCV感染伴有低滴度病毒血症，缺乏临床疾病，但有强烈的中和抗体应答。JCV的第一个完整的序列报告了三个独立的分离株，并观察到一个相对较高的水平的氨基酸序列保守性，即使是分离的病毒相隔57年，表明该病毒是在相对进化停滞。JCV对小鼠和猴具有高度感染性，这些动物，特别是小鼠，是进一步研究的有用实验宿主。
Jamestown Canyon virus (JCV), family Bunyaviridae, is a mosquito-borne pathogen endemic in the United States and Canada that can cause encephalitis in humans and is considered an emerging threat to public health. The virus is genetically similar to Inkoo virus circulating in Europe, suggesting that much of the northern hemisphere contains JCV or similar variants. We have completed the sequence of three isolates of JCV collected in geographically diverse locations over a 57 year time span. The nucleotide identity for the three strains is 90, 83, and 85% for the S, M, and L segments respectively whereas the percent identify for the predicted amino acid sequences of the N, NSS, M poly, GN, NSM, GC, and L proteins was 97, 91, 94, 98, 91, 94, and 97%, respectively. In Swiss Webster mice, each JCV isolate exhibits low neuroinvasiveness but high infectivity. Two of the three JCV isolates were highly neurovirulent after IC inoculation whereas one isolate, JCV/03/CT, exhibited low neurovirulence. In rhesus monkeys, JCV infection is accompanied by a low-titered viremia, lack of clinical disease, but a robust neutralizing antibody response. The first complete sequence of JCV is reported for three separate isolates, and a relatively high level of amino acid sequence conservation was observed even for viruses isolated 57 years apart indicating that the virus is in relative evolutionary stasis. JCV is highly infectious for mice and monkeys, and these animals, especially mice, represent useful experimental hosts for further study.