Epidemiological surveillance of human enteric viruses by monitoring of different environmental matrices
Epidemiological surveillance of human enteric viruses by monitoring of different environmental matrices
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DOI：
10.2166/wst.2006.475
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发表时间：
2006-01-01
影响因子：
2.7
通讯作者：
Casini, B.
中科院分区：
文献类型：
--
作者：
Carducci, A.;Verani, M.;Casini, B.
为了研究在临床标本中发现的病毒与在不同环境基质中发现的病毒之间的可能关系，在2004年5月至2005年4月期间，收集了胃肠炎病例的粪便样本，并每月监测未经处理的废水、河水、海水和贻贝。考虑用于环境监测的病毒有腺病毒、轮状病毒、肠道病毒、诺如病毒、甲型肝炎病毒(HAV)和扭矩Teno病毒(TTV)，采用聚合酶链式反应和逆转录聚合酶链式反应进行检索，并经基因测序证实。还测定了粪便大肠菌群和体细胞大肠杆菌噬菌体计数。病例监测在255个粪便样本中检出45个阳性(17.6%)，而56个环境样本中有35个(62.5%)至少对一种考虑的病毒呈阳性。在胃肠炎病例的粪便和水中检测到相同的病毒株是可能的，腺病毒和轮状病毒也是环境基质中的优势病毒，因此可以作为风险评估的参考。
In the aim of studying possible relations between viruses detected in clinical specimens and the ones found in different environmental matrices, in the period May 2004 to April 2005, the collection of faecal samples from gastroenteritis cases and the monthly monitoring of raw and treated wastewater, river water, seawater and mussels were carried out. The viruses considered for environmental monitoring were adenovirus, rotavirus, enterovirus, norovirus, hepatitis A virus (HAV) and Torque teno virus (TTV): they were searched for with PCR and RT-PCR and confirmed by gene sequencing. Faecal coliforms and somatic coliphages' counts were also determined. The surveillance of case detected 45 positive faecal samples out of 255 (17.6%) while 35 of 56 environmental samples (62.5%) resulted positive for at least one of the considered viruses. The detection of the same viral strain in the faeces of gastroenteritis cases and in water was possible for adenovirus and rotavirus, which were also predominant in environmental matrices; thus they could be considered as a reference for risk assessment.