Drosophila exocyst components Sec5, Sec6, and Sec15 regulate DE-cadherin trafficking from recycling endosomes to the plasma membrane
Drosophila exocyst components Sec5, Sec6, and Sec15 regulate DE-cadherin trafficking from recycling endosomes to the plasma membrane
复制标题
DOI：
10.1016/j.devcel.2005.07.013
复制
发表时间：
2005-09-01
影响因子：
11.8
通讯作者：
Bellaïche, Y
中科院分区：
文献类型：
--
作者：
Langevin, J;Morgan, MJ;Bellaïche, Y
E-钙粘蛋白-连环蛋白复合物在上皮细胞-细胞粘附、极化和形态发生中起关键作用。本文分析了果蝇E-钙粘蛋白（DE-Cad）的定位机制。果蝇外囊组分sec 5、sec 6和sec 15在上皮细胞中的功能丧失导致DE-Cad在扩大的Rab 11再循环内体隔室中积累，并抑制DE-Cad向膜的递送。此外，Rab 11和Armadillo分别与外囊组分Sec 15和Sec 10相互作用。我们的研究结果支持一个模型，即外囊调节DE-钙粘蛋白的贩运，从回收内体的网站上的上皮细胞膜上的犰狳位于。
The E-Cadherin-catenin complex plays a critical role in epithelial cell-cell adhesion, polarization, and morphogenesis. Here, we have analyzed the mechanism of Drosophila E-Cadherin (DE-Cad) localization. Loss of function of the Drosophila exocyst components sec5, sec6, and sec15 in epithelial cells results in DE-Cad accumulation in an enlarged Rab11 recycling endosomal compartment and inhibits DE-Cad delivery to the membrane. Furthermore, Rab11 and Armadillo interact with the exocyst components Sec15 and Sec10, respectively. Our results support a model whereby the exocyst regulates DE-Cadherin trafficking, from recycling endosomes to sites on the epithelial cell membrane where Armadillo is located.