Cytosolic receptor for estradiol in the corpus luteum of the ewe: variation throughout the estrous cycle and distribution between large and small steroidogenic cell types.
Cytosolic receptor for estradiol in the corpus luteum of the ewe: variation throughout the estrous cycle and distribution between large and small steroidogenic cell types.
复制标题
母羊黄体中雌二醇的胞质受体：整个动情周期的变化以及大和小类固醇生成细胞类型之间的分布。
DOI：
10.1095/biolreprod31.5.967
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发表时间：
1984
影响因子：
3.6
通讯作者：
Niswender,GD
中科院分区：
文献类型：
--
作者：
Glass,JD;Fitz,TA;Niswender,GD
本研究的目的是：1）测定绵羊黄体细胞内雌二醇-17 β受体（E2受体）在整个发情周期中的浓度; 2）测定大、小类固醇激素合成和非类固醇激素合成黄体细胞中E2受体的含量。我们的数据证实了存在一个饱和的，高亲和力的受体特异性雌激素在绵羊黄体的胞浆部分。在发情后第4、6、8、12和16天收集的黄体中测定E2受体的浓度，在发情后第4天最低（6.9 ± 0.5fmol/mg蛋白），在发情后第8天最高（19.0 ± 2.8fmol/mg蛋白）（P<0.05）。第12天浓度略有降低（11.8 ± 3.1 fmol/mg蛋白），但第16天再次升高（18.0 ± 4.6 fmol/mg蛋白）。第5天与第15天之间的亲和力常数无差异（P<0.3）。在第10天，大的类固醇生成细胞含有比小的类固醇生成细胞高3.5倍的胞质E2受体浓度（27.7 ± 5.8 vs. 7.9 ± 4.0 fmol/mg蛋白; P<0.05）。E2受体在大细胞和小细胞中的亲和力常数无明显差异。在整个动情周期的细胞溶质E2受体的浓度的差异和较高的浓度的E2受体在大类固醇生成细胞表明，雌二醇可能在调节黄体功能中发挥作用。
This study was designed to:1) determine the concentration of the cytosolic receptor for estradiol-17β(E2receptor) in the ovine corpus luteum throughout the estrous cycle and2) determine the contents of E2receptor in large and small steroidogenic and nonsteroidogenic luteal cells. Our data confirm the existence of a saturable, high-affinity receptor specific for estrogen in the cytosol fraction of ovine corpus luteum. The concentration of E2receptor was assessed in corpora lutea collected at Days 4, 6, 8, 12 and 16 postestrus; it was lowest at Day 4 (6.9 ± 0.5 fmol/mg protein) and maximal (19.0 ± 2.8 fmol/mg protein) at Day 8 (P<0.05). The concentration was decreased somewhat at Day 12 (11.8 ± 3.1 fmol/mg protein), but was higher again at Day 16 (18.0 ± 4.6 fmol/mg protein). The affinity constants were not different between Days 5 and 15 (P<0.3). The large steroidogenic cells contained a 3.5× higher concentration of cytosolic E2receptor than did the small steroidogenic cells at Day 10 (27.7 ± 5.8 vs. 7.9 ± 4.0 fmol/mg protein; P<0.05). There was no apparent difference between the affinity constants of the E2receptor in large and small cells. The differences in concentration of cytosolic E2receptor throughout the estrous cycle and the higher concentrations of the E2receptor in the large steroidogenic cells suggests that estradiol may play a role in regulating luteal function.