Pm67, a new powdery mildew resistance gene transferred from Dasypyrum villosum chromosome 1V to common wheat (Triticum aestivum L.)
Pm67, a new powdery mildew resistance gene transferred from Dasypyrum villosum chromosome 1V to common wheat (Triticum aestivum L.)
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DOI：
10.1016/j.cj.2020.09.012
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发表时间：
2020-11
期刊：
影响因子：
6.6
通讯作者：
Ruiqi Zhang;Chuanxi Xiong;Huanqing Mu;R. Yao;Xiangru Meng;L. Kong;Liping Xing;Jizhong Wu
中科院分区：
文献类型：
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作者：
Ruiqi Zhang;Chuanxi Xiong;Huanqing Mu;R. Yao;Xiangru Meng;L. Kong;Liping Xing;Jizhong Wu
白粉病，由生物营养真菌Blumeria graminisf引起。小麦赤霉病（Bgt）是一种严重威胁小麦生产的全球性病害。为了探索新的抗白粉病基因，以小麦-簇毛麦1号V#5（1D）二体代换系NAU 1813（2n= 42）为材料，通过染色体1V#5和1D之间的重组，获得了一个新的抗白粉病基因。以NAU 1813/NAU 0686杂种F1代的1V#5和1D双单体自交系为材料，选育出t1VS#5双端体异附加系NAU 1815、t1VL#5双端体异附加系NAU 1816、纯合T1 DL·1VS#5易位系NAU 1817和纯合T1 DS·1VL#5易位系NAU 1818。通过荧光原位杂交、基因组素原位杂交、1V特异性标记分析和不同发育时期的白粉病试验对它们进行了鉴定。将一个新的抗白粉病基因Pm 67定位于1VS#5的末端（FL 0.70-1.00）。带有Pm 67的株系表现出苗期免疫和成株期的组织分化反应。Pm 67株系的叶鞘、茎和穗仍具有免疫力，但叶片表现出较低的感病程度。显微观察表明，多数侵入尝试都因叶鞘上的乳突而停止，且成株期在感病叶片上菌落不能形成分生孢子，说明Pm 67株系的防御层是组织分化的。T1 DL·1VS#5易位系NAU 1817为小麦抗白粉病育种提供了新的种质资源。
Powdery mildew, caused by the biotrophic fungusBlumeria graminisf. sp.tritici(Bgt), is a global disease that poses a serious threat to wheat production. To explore additional resistance gene, a wheat-Dasypyrum villosum1 V#5 (1D) disomic substitution line NAU1813 (2n= 42) with high level of seedling resistance to powdery mildew was used to generate the recombination between chromosomes 1 V#5 and 1D. Four introgression lines, including t1VS#5 ditelosomic addition line NAU1815, t1VL#5 ditelosomic addition line NAU1816, homozygous T1DL·1VS#5 translocation line NAU1817, and homozygous T1DS·1VL#5 translocation line NAU1818 were developed from the selfing progenies of 1 V#5 and 1D double monosomic line that derived from F1hybrids of NAU1813/NAU0686. All of them were characterized by fluorescencein situhybridization, genomicin situhybridization, 1 V-specific markers analysis, and powdery mildew tests at different developmental stages. A new powdery mildew resistance gene namedPm67was physically located in the terminal bin (FL 0.70–1.00) of 1VS#5. Lines withPm67exhibited seedling stage immunity and tissue-differentiated reactions at adult plant stage. The sheaths, stems, and spikes of thePm67line were still immune, but the leaves showed a low degree of susceptibility. Microscopic observation showed that most penetration attempts were stopped in association with papillae on the sheath, and colonies cannot form conidia on the susceptible leaf ofPm67line at adult plant stage, suggesting that the defence layers of thePm67line is tissue-differentiated. Thus, the T1DL·1VS#5 translocation line NAU1817 provides a new germplasm in wheat breeding for improvement of powdery mildew resistance.