Acupuncture inhibits ferric iron deposition and ferritin-heavy chain reduction in an MPTP-induced parkinsonism model
Acupuncture inhibits ferric iron deposition and ferritin-heavy chain reduction in an MPTP-induced parkinsonism model
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DOI：
10.1016/j.neulet.2008.11.049
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发表时间：
2009-01-30
影响因子：
2.5
通讯作者：
Lim, Sabina
中科院分区：
文献类型：
--
作者：
Choi, Yeong-Gon;Park, Jae-Hyun;Lim, Sabina
本研究采用1-甲基-4-苯基-1，2，3，6-四氢吡啶(MPTP)诱导的帕金森病小鼠模型，观察针刺对铁相关氧化损伤的影响。采用MPTP(20 mg/kg，1次/d)连续30d腹腔注射建立小鼠帕金森病模型，针刺LR3(太冲)、GB34(阳陵泉)穴，每隔48h进行一次针刺。针刺可抑制MPTP神经毒性引起的酪氨酸羟化酶(TH)和多巴胺转运体(DAT)免疫活性下降。由于慢性负荷MPTP，黑质(SN)中铁离子(Fe3+)的存在显著增加，而铁蛋白重链(F-H)显著减少。但针刺可抑制MPTP引起的铁含量的升高和F-H的降低。此外，MPTP和针灸治疗后，亚铁和铁蛋白轻链(F-L)的存在没有因治疗而改变。F-H基因的表达也不受影响。这些结果表明，针刺可抑制铁相关的氧化损伤，并可防止MPTP模型中铁代谢的有害改变。(C)2008爱思唯尔爱尔兰有限公司。保留所有权利。
This study investigated the effect of acupuncture on iron-related oxidative damage in a mouse model designed as a 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP)-induced parkinsonism model. To generate the chronic parkinsonism model, mice were intraperitoneally injected with MPTP (20 mg/kg, one daily injection) for 30 days and acupuncture was performed at acupoints LR3 (Taichong) and GB34 (Yanglingquan) at 48 h intervals. Acupuncture inhibited decreases in the immunoreactivities of tyrosine hydroxylase (TH) and dopamine transporter (DAT) that occurred as a result of MPTP neurotoxicity. The presence of ferric iron (Fe3+), but not ferrous iron (Fe2+), was strongly increased in the substantia nigra (SN) as a result of chronic loading of MPTP, whereas the ferritin-heavy chain (F-H) was significantly decreased. However, acupuncture treatment inhibited the increase in ferric iron and the decrease in the F-H that was induced by MPTP. Additionally, treatment with MPTP and acupuncture caused no changes in the presence of ferrous iron and ferritin-light chain (F-L) as a result of the treatments. The mRNA of F-H was also not affected. These results suggest that acupuncture may inhibit iron-related oxidative damage and may prevent the deleterious alteration of iron metabolism in the MPTP model. (C) 2008 Elsevier Ireland Ltd. All rights reserved.