RETENTION OF LEUKOCYTES IN CAPILLARIES - ROLE OF CELL-SIZE AND DEFORMABILITY
RETENTION OF LEUKOCYTES IN CAPILLARIES - ROLE OF CELL-SIZE AND DEFORMABILITY
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DOI：
10.1152/jappl.1990.69.5.1767
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发表时间：
1990-11-01
影响因子：
3.3
通讯作者：
WORTHEN, GS
中科院分区：
文献类型：
--
作者：
DOWNEY, GP;DOHERTY, DE;WORTHEN, GS
循环中的白细胞与边缘池处于动态平衡，边缘池被认为主要位于肺毛细血管内。循环白细胞的平均直径（6 - 8 μ m）和肺毛细血管的平均直径（约10 μ m）之间的尺寸差异是显著的。5.5μ m）迫使细胞变形以通过毛细血管床。因此，我们研究了细胞大小和变形性的生物物理特性决定了肺中白细胞滞留的差异的假设。通过聚碳酸酯过滤器（5 μ m孔径）对人中性粒细胞、淋巴细胞、单核细胞、血小板和红细胞的过滤性能进行比较，发现最大的白细胞（中性粒细胞和单核细胞）被最大程度地保留，而较小的细胞（淋巴细胞和血小板）被最小程度地保留。未分化的HL-60细胞，直径大于其分化的对应物，也保留在更大的程度上，证实细胞大小是一个重要的决定因素，保留在这些模型毛细血管。然而，与直径相似的中性粒细胞相比，单核细胞被保留的程度更大，这表明单核细胞可能比中性粒细胞更不易变形。为了检验这一假设，变形性直接使用细胞扑克测量。单核细胞是最硬的，中性粒细胞是最软的，淋巴细胞居中。中性粒细胞的戊二醛处理显著增加了它们的硬度，降低了它们通过体外滤器孔和兔肺微血管的能力，而不改变它们的粘附特性或大小。这些观察结果支持白细胞的生物物理特性（大小和变形性）部分决定其通过肺毛细血管的能力，并可能决定其边缘池的大小的假设。
Leukocytes within the circulation are in dynamic equilibrium with a marginated pool, thought to reside mainly within the pulmonary capillaries. The size discrepancy between the mean diameter of circulating leukocytes (6-8 .mu.m) and that of the pulmonary capillaries (.apprxeq. 5.5 .mu.m) forces the cells to deform in order to transit the capillary bed. Consequently, we investigated the hypothesis that the biophysical properties of cell size and deformability determined differential leukocyte retention in the lung. Comparison of the filtration properties of human neutrophils, lymphocytes, monocytes, platelets, and erythrocytes through polycarbonate filters (5-.mu.m pore diameter) revealed that the largest leukocytes (neutrophils and monocytes) were retained to the greatest extent and the smaller cells (lymphocytes and platelets) the least. Undifferentiated HL-60 cells, of greater diameter than their differentiated counterparts, were also retained to a greater extent, confirming that cell size was one important determinant of retention in these model capillaries. However, compared with neutrophils, which are of similar diameter, monocytes were retained to a greater extent, suggesting that monocytes might be less deformable than neutrophils. To test this hypothesis, deformability was measured directly using the cell poker. Monocytes were found to be the stiffest, neutrophils the softest, and lymphocytes intermediate. Glutaraldehyde treatment of neutrophils markedly increased their stiffness and decreased their ability to transit the pores of the filters in vitro and the pulmonary microvasculature of rabbits without changing their adhesive properties or size. These observations support the hypothesis that biophysical properties of leukocytes (size and deformability) determine in part their ability to transit the pulmonary capillaries and may determine the magnitude of their marginated pools.