Filter-exchange PGSE NMR determination of cell membrane permeability
Filter-exchange PGSE NMR determination of cell membrane permeability
复制标题
DOI：
10.1016/j.jmr.2009.07.015
复制
发表时间：
2009-10-01
影响因子：
2.2
通讯作者：
Topgaard, Daniel
中科院分区：
文献类型：
--
作者：
Aslund, Ingrid;Nowacka, Agnieszka;Topgaard, Daniel
介绍了一种新的PGSE NMR序列，用于测量活细胞跨质膜的扩散运输。“扩散滤波器”和可变混合时间在标准PGSE块之前，用于NMR信号的扩散编码。该过滤器是一个PGSE块优化选择性去除磁化的细胞外水。随着混合时间的增加，细胞内和细胞外组分接近其平衡分数群体。只需几分钟的仪器时间就可以测量交换率。在+5至+32 ℃的温度范围内研究了饥饿酵母细胞质膜上的水交换。(C)2009 Elsevier Inc. All rights reserved.
A new PGSE NMR sequence is introduced for measuring diffusive transport across the plasma membrane of living cells. A "diffusion filter" and a variable mixing time precedes a standard PGSE block for diffusion encoding of the NMR signal. The filter is a PGSE block optimized for selectively removing the magnetization of the extracellular water. With increasing mixing time the intra- and extracellular components approach their equilibrium fractional populations. The rate of exchange can be measured using only a few minutes of instrument time. Water exchange over the plasma membrane of starved yeast cells is studied in the temperature range +5 to +32 degrees C. (C) 2009 Elsevier Inc. All rights reserved.