Phytostimulant properties of highly stable silver nanoparticles obtained with saponin extract from Chenopodium quinoa
Phytostimulant properties of highly stable silver nanoparticles obtained with saponin extract from Chenopodium quinoa
复制标题
用藜麦皂苷提取物获得的高度稳定的银纳米颗粒的植物刺激特性
DOI：
10.1002/jsfa.10529
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发表时间：
2020
影响因子：
4.1
通讯作者：
Cabrera‐Barjas, Gustavo
中科院分区：
文献类型：
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作者：
Segura, Rodrigo;Vásquez, Gustavo;Colson, Emmanuel;Gerbaux, Pascal;Frischmon, Caroline;Nesic, Aleksandra;García, Danni E;Cabrera‐Barjas, Gustavo
藜麦（Chenopodium quinoaWilld）是安第斯山脉的原始伪谷物，具有很高的营养价值。在藜麦加工过程中，获得大量富含皂苷的外壳副产物。藜皂苷具有生物活性，可用于各种农业用途。银纳米颗粒在农业作物病害管理中越来越受到关注。本研究以藜麦皮皂苷提取物（QE）为原料，采用一种可持续的绿色合成方法合成了银纳米颗粒，并对其进行了表征。16皂苷同系物成功地确定和定量。将所得QE用作银离子的还原剂，在温和条件下合成银纳米颗粒（QEAgNPs）。通过透射电子显微镜（TEM）、紫外-可见光谱（UV-可见）、能量色散X射线（EDS）、zeta电位和具有衰减全反射的傅里叶变换红外光谱（FTIR-ATR）研究Ag纳米颗粒的形貌、粒径和稳定性。紫外-可见光谱测量证实了在QE存在下形成银纳米颗粒，估计颗粒尺寸在5和50 nm之间的范围内。根据zeta电位值，形成高度稳定的纳米颗粒。还在萝卜种子生物测定中测试QE和QEAgNP（200-1000 μg/mL）以评估其植物毒性。种子发芽试验表明，QEAgNPs具有剂量依赖性的方式对萝卜种子的植物刺激作用，并没有观察到QE和QEAgNPs. CONCLUSION银纳米粒子通过所谓的“绿色”的方法获得可以被认为是一个很好的候选人，在农业部门的种子处理，或作为叶面喷雾剂和植物生长促进剂的应用。© 2020化学工业学会
BACKGROUNDQuinoa (Chenopodium quinoaWilld) is an Andean original pseudocereal with high nutritional value. During quinoa processing, large amounts of saponin‐rich husks byproducts are obtained. Quinoa saponins, which are biologically active, could be used for various agriculture purposes. Silver nanoparticles have increasingly attracted attention for the management of crop diseases in agriculture. In this work, silver nanoparticles are synthesized by a sustainable and green method, using quinoa husk saponin extract (QE) to evaluate their potential for application in agriculture as biostimulants.RESULTSQuinoa extract was obtained and characterized by liquid chromatography with tandem mass spectrometry (LC–MS/MS). Sixteen saponin congeners were successfully identified and quantified. The QE obtained was used as a reducing agent for silver ions to synthesize silver nanoparticles (QEAgNPs) under mild conditions. The morphology, particle size, and stability of Ag nanoparticles were investigated by transmission electron microscopy (TEM), ultraviolet–visible spectroscopy (UV‐visible), energy‐dispersive X‐ray (EDS), zeta potential, and Fourier transform infrared spectroscopy with attenuated total reflection (FTIR‐ATR). Ultraviolet–visible spectroscopy measurements confirmed the formation of silver nanoparticles in the presence of QE, with estimated particle sizes in a range between 5 and 50 nm. According to the zeta potential values, highly stable nanoparticles were formed. The QE and QEAgNPs (200–1000 μg/mL) were also tested in radish seed bioassay to evaluate their phytotoxicity. The seed germination assays revealed that QEAgNPs possessed a phytostimulant effect on radish seeds in a dose‐dependent manner, and no phytotoxicity was observed for both QE and QEAgNPs.CONCLUSIONSilver nanoparticles obtained by a so‐called ‘green’ method could be considered as good candidates for application in the agricultural sector for seed treatment, or as foliar sprays and plant‐growth‐promoters. © 2020 Society of Chemical Industry