Considerations when investigating lncRNA function in vivo.
Considerations when investigating lncRNA function in vivo.
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DOI：
10.7554/elife.03058
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发表时间：
2014-08-14
期刊：
影响因子：
7.7
通讯作者：
Ponting CP
中科院分区：
文献类型：
--
作者：
Bassett AR;Akhtar A;Barlow DP;Bird AP;Brockdorff N;Duboule D;Ephrussi A;Ferguson-Smith AC;Gingeras TR;Haerty W;Higgs DR;Miska EA;Ponting CP
虽然一小部分动物长链非编码RNA（lncRNA）对体外检测的细胞过程有已知的影响，但它们在整个发育、分化和疾病中对正常细胞过程的贡献程度仍然不太清楚。由整个基因组位点缺失引起的表型不能明确地归因于lncRNA本身的丢失或其他重叠DNA调控元件的相关丢失。顺式效应和反式效应之间的区别也常常是有问题的。我们根据lncRNA分子机制的不同模型讨论了当前研究lncRNA体内功能的技术的优势和挑战，并反思了突变lncRNA基因座的实验设计。这些考虑应该有助于进一步研究这些基因组的转录产物。DOI：http://dx.doi.org/10.7554/eLife.03058.001网站
Although a small number of the vast array of animal long non-coding RNAs (lncRNAs) have known effects on cellular processes examined in vitro, the extent of their contributions to normal cell processes throughout development, differentiation and disease for the most part remains less clear. Phenotypes arising from deletion of an entire genomic locus cannot be unequivocally attributed either to the loss of the lncRNA per se or to the associated loss of other overlapping DNA regulatory elements. The distinction between cis- or trans-effects is also often problematic. We discuss the advantages and challenges associated with the current techniques for studying the in vivo function of lncRNAs in the light of different models of lncRNA molecular mechanism, and reflect on the design of experiments to mutate lncRNA loci. These considerations should assist in the further investigation of these transcriptional products of the genome. DOI: http://dx.doi.org/10.7554/eLife.03058.001