Coriander Leaf Extract Exerts Antioxidant Activity and Protects Against UVB-Induced Photoaging of Skin by Regulation of Procollagen Type I and MMP-1 Expression
Coriander Leaf Extract Exerts Antioxidant Activity and Protects Against UVB-Induced Photoaging of Skin by Regulation of Procollagen Type I and MMP-1 Expression
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DOI：
10.1089/jmf.2013.2999
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发表时间：
2014-09-01
影响因子：
2.4
通讯作者：
Kim, Sun Yeou
中科院分区：
文献类型：
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作者：
Hwang, Eunson;Lee, Do-Gyeong;Kim, Sun Yeou
紫外线（UV）辐射引起皮肤的光损伤，这反过来导致真皮细胞外基质的耗尽和皮肤结构的慢性改变。皮肤皱纹与胶原蛋白合成和基质金属蛋白酶-1（MMP-1）活性有关。芫荽（芫荽叶，芫荽叶; CS）已被用作治疗糖尿病、高脂血症、肝病和癌症的草药。在这项研究中，我们研究了CS乙醇提取物（CSE）是否对UVB诱导的皮肤光老化在正常人皮肤成纤维细胞（NHDF）在体外和在无毛小鼠的皮肤在体内的保护作用。采用气相色谱-质谱联用技术测定了CSE的主要成分亚麻酸。我们用ELISA法检测了UVB照射后NHDF细胞中I型前胶原和MMP-1的细胞水平。UVB照射后用CSE处理的NHDF细胞比未处理的细胞表现出更高的I型前胶原产生和更低的MMP-1水平。我们发现，转录因子激活蛋白-1（AP-1）的活性也被CSE处理抑制。我们测量了表皮厚度，真皮胶原纤维密度，和前胶原I型和MMP-1水平在光老化小鼠皮肤在体内使用组织学染色和蛋白质印迹分析。我们的研究结果表明，CSE治疗的小鼠比未治疗的小鼠具有更薄的表皮层和更致密的真皮胶原纤维。在分子水平上，进一步证实了CSE处理的小鼠比未处理的小鼠具有更低的MMP-1水平和更高的I型前胶原水平。我们的结果支持了C. sativum L.防止皮肤光老化。
Ultraviolet (UV) radiation causes photodamage to the skin, which, in turn, leads to depletion of the dermal extracellular matrix and chronic alterations in skin structure. Skin wrinkles are associated with collagen synthesis and matrix metalloproteinase-1 (MMP-1) activity. Coriandrum sativum L. (coriander leaf, cilantro; CS) has been used as a herbal medicine for the treatment of diabetes, hyperlipidemia, liver disease, and cancer. In this study, we examined whether CS ethanol extract (CSE) has protective effects against UVB-induced skin photoaging in normal human dermal fibroblasts (NHDF) in vitro and in the skin of hairless mice in vivo. The main component of CSE, linolenic acid, was determined by gas chromatography-mass spectroscopy. We measured the cellular levels of procollagen type I and MMP-1 using ELISA in NHDF cells after UVB irradiation. NHDF cells that were treated with CSE after UVB irradiation exhibited higher procollagen type I production and lower levels of MMP-1 than untreated cells. We found that the activity of transcription factor activator protein-1 (AP-1) was also inhibited by CSE treatment. We measured the epidermal thickness, dermal collagen fiber density, and procollagen type I and MMP-1 levels in photo-aged mouse skin in vivo using histological staining and western blot analysis. Our results showed that CSE-treated mice had thinner epidermal layers and denser dermal collagen fibers than untreated mice. On a molecular level, it was further confirmed that CSE-treated mice had lower MMP-1 levels and higher procollagen type I levels than untreated mice. Our results support the potential of C. sativum L. to prevent skin photoaging.