CD133+ niches and single cells in glioblastoma have different phenotypes
CD133+ niches and single cells in glioblastoma have different phenotypes
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DOI：
10.1007/s11060-010-0488-y
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发表时间：
2011-08-01
影响因子：
3.9
通讯作者：
Kristensen, Bjarne Winther
中科院分区：
文献类型：
--
作者：
Christensen, Karina;Schroder, Henrik Daa;Kristensen, Bjarne Winther
推定的 CD133(+) 脑肿瘤干细胞已被证明位于微环境中并且作为单个细胞。这是第一项根据定位深入了解胶质母细胞瘤中 CD133(+) 细胞不同表型的研究。石蜡切片采用 CD133 和候选干细胞标记物 Sox2、Bmi-1、EGFR、podoplanin 和 nestin、增殖标记物 Ki67 以及内皮细胞标记物 CD31、CD34 和 VWF 进行双重免疫荧光染色。细胞计数显示，与CD133(+)单细胞相比，微环境中的CD133(+)细胞的Sox2、EGFR和巢蛋白表达显着较高，但Ki67标记指数仅为3%，而CD133(+)单细胞为14%。仅在微环境或CD133(-)肿瘤区域发现低内皮细胞标志物表达，而发现43%的CD133(+)/CD31(+)和25%的CD133(+)/CD34(+)单细胞。 CD133(+)微环境内的CD133(+)血管比微环境外的CD133(+)血管增殖较少，并且Bmi-1(+)的情况更常见。总之，根据原位定位存在不同的CD133(+)细胞表型，并且CD133(+)血管的表型也根据定位而变化。 CD133(+) 微环境包含增殖指数低于 CD133(+) 单细胞的干细胞样细胞，其内皮分化特征表明其在血管生成中发挥作用。
Putative CD133(+) brain tumor stem cells have been shown to be located in niches and as single cells. This is the first study providing insight into the different phenotypes of CD133(+) cells in glioblastoma according to localization. Paraffin sections were stained by double immunofluorescence with CD133 and the candidate stem cell markers Sox2, Bmi-1, EGFR, podoplanin and nestin, the proliferation marker Ki67 and the endothelial cell markers CD31, CD34, and VWF. Cell counting showed that the CD133(+) cells in the niches had a significantly higher expression of Sox2, EGFR and nestin compared to CD133(+) single cells, but only a 3% Ki67 labeling index versus 14% found for CD133(+) single cells. Only low endothelial cell marker expression was found in the niches or the CD133(-) tumor areas, while 43% CD133(+)/CD31(+) and 25% CD133(+)/CD34(+) single cells were found. CD133(+) blood vessels within CD133(+) niches were less proliferative and more often Bmi-1(+) than CD133(+) blood vessels outside niches. In conclusion, different CD133(+) cell phenotypes exist according to the in situ localization, and also the phenotype of CD133(+) blood vessels vary according to the localization. CD133(+) niches contain stem-like cells with a lower proliferation index than CD133(+) single cells, which have an endothelial differentiation profile suggesting a role in angiogenesis.