Isolation of Bacillus subtilis: screening for aflatoxins B1, M1, and G1 detoxification
Isolation of Bacillus subtilis: screening for aflatoxins B1, M1, and G1 detoxification
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DOI：
10.1007/s00217-011-1463-3
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发表时间：
2011-06-01
影响因子：
3.3
通讯作者：
Ji, Cheng
中科院分区：
文献类型：
--
作者：
Gao, Xin;Ma, Qiugang;Ji, Cheng
黄曲霉毒素在世界各地的谷物中普遍存在，对食品工业和动物生产造成重大威胁。本研究旨在从动物肠道和土壤中寻找能同时转化黄曲霉毒素B-1、M-1和G（1）的枯草芽孢杆菌。此外，选择的B.枯草芽孢杆菌能抑制病原菌的生长，并能抵抗模拟肠道环境中的不利条件。采用高效液相色谱法测定黄曲霉毒素浓度的降低。B。鱼内脏来源的枯草杆菌ANSB 060对黄曲霉毒素B-1、M-1和G（1）的降解率分别为81.5%、60%和80.7%。此外，结果表明黄曲霉毒素降解活性主要存在于ANSB 060的培养上清中，而不是其细胞或细胞提取物中。ANSB 060对大肠杆菌、鼠伤寒沙门氏菌、金黄色葡萄球菌有一定的抗菌活性，对模拟肠道环境有一定的抵抗力。研究表明ANSB 060降解黄曲霉毒素具有很大的工业应用潜力。
Aflatoxins are commonly found in cereals worldwide and bring significant threats to the food industry and animal production. The aim of this research was to search for Bacillus subtilis from animals' guts and soil capable of transforming aflatoxin B-1, M-1, and G(1) simultaneously. In addition, the selected B. subtilis can inhibit the growth of pathogen and resist to unfavorable conditions within simulated gut environments. High-performance liquid chromatography was used to determine the reduction in aflatoxin concentrations. B. subtilis ANSB060 from fish gut had the strongest ability to detoxify toxins, and percentages of aflatoxin B-1, M-1, and G(1) degradation were 81.5%, 60%, and 80.7%, respectively. Moreover, the results implied that the activity of aflatoxins degradation was mainly in the culture supernatant of ANSB060 rather than its cells or cell extracts. And ANSB060 showed antimicrobial activities against Escherichia coli, Salmonella typhimurium, Staphylococcus aureus and resistance to the simulated gut environments. This research indicated that the degradation of aflatoxins by ANSB060 could have a great potential in industrial applications.