Regulation of defense-related enzymes associated with bacterial spot resistance in tomato
Regulation of defense-related enzymes associated with bacterial spot resistance in tomato
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DOI：
10.1007/bf02981327
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发表时间：
2008-03-01
期刊：
影响因子：
1.4
通讯作者：
Umesha, S.
中科院分区：
文献类型：
--
作者：
Kavitha, R.;Umesha, S.
对20个番茄品种进行了黄单胞菌细菌性斑点病抗性鉴定。病原菌感染和未感染的田间条件下的水泡菌。通过人工接种X.axonopodis PV进行筛选。对4周龄番茄幼苗接种发泡菌悬浮液，观察其典型症状。根据发病情况，将苗木分为高抗、抗病、感病和高感4个品种。对番茄品种进行了抗病相关酶、总酚和木质素含量的筛选。用一个中等感病番茄品种估计了所有这些酶的时间模式。以3个不同番茄品种为材料，对其侵染后的过氧化物酶(POX)和多酚氧化酶(PPO)活性进行了自然PAGE分析。根据病原菌侵染时这些酶的诱导量，可以预测番茄品种在田间条件下的发病情况。显著(P
Twenty tomato (Solanum lycopersicon) cultivars were screened for resistance against bacterial spot disease incited by Xanthomonas axonopodis pv. vesicatoria under field conditions with and without pathogen infection. Screening was done by artificially inoculating a X. axonopodis pv. vesicatoria suspension to 4-week-old tomato seedlings and observing them for typical symptoms of the disease. Seedlings were categorized into highly resistant, resistant, susceptible and highly susceptible cultivars on the basis of disease incidence. Tomato cultivars were screened for defense-related enzymes, total phenols and lignin contents. The temporal patterns of all these enzymes were estimated with a moderately susceptible tomato cultivar. Native PAGE analysis of both peroxidase (POX) and polyphenol oxidase (PPO) was carried out for the time course of enzyme activities and also by selecting three different tomato cultivars, following infection with the pathogen. Based on the inducible amounts of these enzymes upon pathogen infection, the tomato cultivars were correlated with the disease incidence under field conditions. A significant (P